We narrowed to 14,581 results for: pET

1. pET19-TdTwt

   Plasmid

   #207466

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTwt (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEThT-RvLEAM

   Plasmid

   #90033

   Purpose

   To express His-tagged RvLEAM in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   RvLEAM
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET30a-HS-Tn5

   Plasmid

   #127916

   Purpose

   Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Hyperstable Tn5
   

   Use

   Tags

   E coli elongation factor Ts and Mxe intein - Chit…

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-pduCDE

   Plasmid

   #110138

   Purpose

   Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

   Insert

   pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET16b-Trx

   Plasmid

   #36944

   Depositor

   Anna Blom

   Article

   King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

   Insert

   Thioredoxin 1 (TXN Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22b-CPDSalI

   Plasmid

   #38251

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28:GFP

   Plasmid

   #60733

   Purpose

   pET28 driving expression of GFP, biobrick part Bba_E0040

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET30a-Tn5

   Plasmid

   #127917

   Purpose

   Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Tn5
   

   Use

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-1∆HisTag_hNMT_Nef

    Plasmid

    #66078

    Purpose

    Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

    Depositor

    Dieter Willbold

    Article

    Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

    Inserts

    NMT (NMT1 Human)
    Nef
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of first 80 amino acids

    Promoter

    T7-lacO

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NylCK-TS_pET28a

    Plasmid

    #215423

    Purpose

    Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK-TS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S111G/A137L

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-HsSENP3

    Plasmid

    #71467

    Purpose

    Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-3c-rhVTN-N

    Plasmid

    #30225

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 Apr 10. ():.

    Insert

    rhVitronectin with N terminus cleavage (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids: 20*-62

    Promoter

    Available since

    May 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LCC-ICCG_pET21b

    Plasmid

    #215408

    Purpose

    Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    LCC-ICCG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F243I/D238C/S283C/Y127G

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET30B-PTEN

    Plasmid

    #20741

    Depositor

    Alonzo Ross

    Article

    Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-SET

    Plasmid

    #234708

    Purpose

    expresses His-tagged human SET in E coli

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETduet+p300HAT

    Plasmid

    #157793

    Purpose

    Expression of p300 HAT domain

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    p300HAT (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lac operator-controlled T7 promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET11a-Z-NspGFP

    Plasmid

    #40729

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    N super positive GFP - Leucine Zipper
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a His6HuR

    Plasmid

    #135943

    Purpose

    Expresses HuR in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    MMLV Reverse Transcriptase
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pET32a-HD25Q

    Plasmid

    #11508

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 25 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 25 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Use

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET20b-delta133HtrA2

    Plasmid

    #14126

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b-hnRNP-H

    Plasmid

    #23020

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-H (HNRNPH1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polymerase

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pET28a-cMSP1E3D1

    Plasmid

    #199772

    Purpose

    Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

    Insert

    cMSP1E3D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Extended cMSP1D1; external His-Tags flanking the …

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge