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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 56,989 results for: %s

Showing: 241 - 260 of 56989 results

  6. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  8. G protein alpha-s-HaloTag

    Plasmid
    #66993
    Purpose
    G protein alpha-s internally tagged with HaloTag and EE epitope
    Depositor
    Catherine Berlot
    Insert
    G-alpha-s-EE-HaloTag (Gnas Rat)
    Use
    Tags
    HaloTag flanked by SGGGGSGGGGS on each side was i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66993-plasmid-map-sequence-id-122192

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  9. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212993-plasmid-map-sequence-id-423196

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  10. pαH-S-RRAR/XBB.1.16

    Plasmid
    #213037
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    s -RRAR.XBB.1.16 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213037-plasmid-map-sequence-id-423206

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  11. pαH-S-RRAR-OMICRON.BA.5

    Plasmid
    #213071
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-RRAR-OMICRON.BA.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685, furin site inta…
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213071-plasmid-map-sequence-id-422361

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  12. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  13. pαH-S-GSAS-OMICRON.BA.2

    Plasmid
    #184829
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi
    Insert
    S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184829-plasmid-map-sequence-id-363836

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  14. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

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  17. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

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  19. pCAGGS-S-B.1.617.2-FLAG

    Plasmid
    #177097
    Purpose
    B.1.617.2 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein B.1.617.2 (S SARS-CoV-2)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177097-plasmid-map-sequence-id-345527

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  20. pCAGGS-S-B.1.1.529-FLAG

    Plasmid
    #180774
    Purpose
    B.1.1.529 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein B.1.1.529
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180774-plasmid-map-sequence-id-355099

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Showing: 241 - 260 of 56989 results