We narrowed to 59,899 results for: %s

1. Plxnb1(S)-AP-His

   Plasmid

   #72001

   Purpose

   Expresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb1 (Plxnb1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sema3d(S)-AP-His

   Plasmid

   #72018

   Purpose

   Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3d (Sema3d Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sema3b(S)-AP-His

   Plasmid

   #72014

   Purpose

   Expresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3b (Sema3b Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-Neo-JAM-A

   Plasmid

   #112965

   Purpose

   To express feline Jam-A transiently in cell lines that do not naturally express the receptor.

   Depositor

   John S. L. Parker

   Article

   Lu et al J Virol. 2018 Mar 28;92(8):e00035-18. do

   Insert

   Junctional Adhesion Molecule type A (JAM-A) (F11R Felis catus)
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX-SHP1C/S-ires-mCD8

   Plasmid

   #183719

   Purpose

   Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Tags

   myc

   Mutation

   C453S

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV phSyn1(S)-tdTomato-WPRE

   Plasmid

   #51506

   Purpose

   Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Available Since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pαH-S-GSAS/XBB.1.5

   Plasmid

   #212991

   Purpose

   "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-GSAS/XBB.1.5 (S )
   

   Tags

   HRV 3C cleavage site (before tags) C terminal on …

   Expression

   Mammalian

   Mutation

   (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Anti-S-tag [N56/9R]

   Plasmid

   #206600

   Purpose

   Mammalian Expression Plasmid of anti-S-tag (Bovine). Derived from hybridoma N56/9.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-S-tag (Bos taurus) recombinant Mouse monoclonal antibody (RNASE1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCD-Casp3-L-tev-S

    Plasmid

    #226230

    Purpose

    Express TEVP-cleavable caspase-3

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    Caspase-3 (CASP3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoMyc rat S-SCAM alpha

    Plasmid

    #40213

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

    Insert

    S-SCAM (Magi2 Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. AAV phSyn1(S)-DreO-bGHpA

    Plasmid

    #50363

    Purpose

    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde and AAV5

    Insert

    DreO recombinase
    

    Use

    AAV

    Tags

    none

    Mutation

    Sequence optimized for expression in mammalian ce…

    Promoter

    hSyn1

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 SARS-CoV-2 S

    Plasmid

    #149329

    Purpose

    Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-S in pAR9 (rep/cap)

    Plasmid

    #174539

    Purpose

    encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

    Depositor

    Casey Maguire

    Article

    Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

    Insert

    VP1 of AAV9 containing the AAV-S 7-mer peptide insert
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV phSyn1(S)-FlpO-bGHpA

    Plasmid

    #51669

    Purpose

    Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde

    Insert

    FlpO recombinase gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. G protein alpha-s-HaloTag

    Plasmid

    #66993

    Purpose

    G protein alpha-s internally tagged with HaloTag and EE epitope

    Depositor

    Catherine Berlot

    Insert

    G-alpha-s-EE-HaloTag (Gnas Rat)
    

    Tags

    HaloTag flanked by SGGGGSGGGGS on each side was i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pαH-S-GSAS-2P-OMICRON

    Plasmid

    #180593

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. G protein alpha-s-YFP

    Plasmid

    #55781

    Purpose

    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    alpha-s-EE YFP (Gnas Rat)
    

    Tags

    EYFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln69 in EYFP (Clontech).…

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. G protein alpha-s-CFP

    Plasmid

    #55793

    Purpose

    G protein alpha-s internally tagged with ECFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-ECFP (Gnas Rat)
    

    Tags

    ECFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    His was substituted for Asn164 in ECFP (Clontech)…

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. G protein alpha-s-GFP

    Plasmid

    #66083

    Purpose

    G protein alpha-s internally tagged with EGFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-EGFP (Gnas Rat)
    

    Tags

    EGFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge