Addgene

We narrowed to 8,312 results for: sgrna

Showing: 241 - 260 of 8312 results

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PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-29

Plasmid

#66203

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA1

Plasmid

#201590

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-4sgRNA

Plasmid

#64242

Purpose

destination vector for four U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP53_sgRNA-1

Plasmid

#88853

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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U6-sgRNAs-Crym

Plasmid

#200067

Purpose

For the knock down of Crym with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-3xsgRNA-Crym

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-GFP

Plasmid

#200068

Purpose

For the knock down of GFP with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-sgRNA-GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

OsU6a

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-2sgRNA

Plasmid

#64240

Purpose

destination vector for two U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Tags

Expression

Mutation

Promoter

U6

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-3sgRNA

Plasmid

#64241

Purpose

destination vector for three U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-1sgRNA

Plasmid

#64239

Purpose

destination vector for one U6x:sgRNA cassette

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLsgRNA-OsU6c

Plasmid

#66197

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

OsU6c

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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