We narrowed to 31,550 results for: Eng;

1. TU#1449_unc-73e_cDNA

   Plasmid

   #82363

   Purpose

   express unc-73e cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-73 e isoform (unc-73 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD

   Plasmid

   #73303

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   E1083Q

   Promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

   Plasmid

   #73299

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   S1079N-E1083Q

   Promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pTH1-SaSrtA-KQ

   Plasmid

   #64982

   Purpose

   Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108Q

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. human pirh2-(138-261)

   Plasmid

   #24882

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   Pirh2 (RCHY1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains aa 138-261.

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

6. human pirh2-(187-261)

   Plasmid

   #24883

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   Pirh2 (RCHY1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains aa 187-261.

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

7. L1-W1036L

   Plasmid

   #15488

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-W1036L (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tryptophan 1036 changed to Leucine

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Human, Synthetic)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3544 pMIG Bcl-2

    Plasmid

    #8793

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV Puro SCRIB WT

    Plasmid

    #88886

    Purpose

    SCRIB WT in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    N/A

    Expression

    Mutation

    N/A

    Promoter

    MSCV promoter

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSFFV_sfCherry3C(1-10)

    Plasmid

    #117482

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3C(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3C(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    5 substitutions (K45R, G52D, T106A, K182R, N194D)…

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pILGFP91

    Plasmid

    #83548

    Purpose

    Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PGK1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHIV-iRFP720-E2A-Luc

    Plasmid

    #104587

    Purpose

    Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

    Depositor

    Antonius Plagge

    Article

    Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

    Insert

    iRFP720-E2A-Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    bi-cistronic

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid

    #117656

    Purpose

    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Inserts

    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYPQAT22

    Plasmid

    #223394

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQAT19

    Plasmid

    #223391

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYPQAT21

    Plasmid

    #223393

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSFFV-SpyTag-sfCherry2(11)-TagBFP

    Plasmid

    #117485

    Purpose

    Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyTag002-GG-sfCherry2(11)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

    Plasmid

    #117484

    Purpose

    Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyCatcher002-6aa-sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge