We narrowed to 29,679 results for: REP

1. pT-GEM(0)

   Plasmid

   #62891

   Purpose

   Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

   Plasmid

   #74289

   Purpose

   pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   H2B-GFP and oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_V5-APEX2-Sec61B

   Plasmid

   #83412

   Purpose

   Expressed N-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-Sec61B (SEC61B Human, Synthetic)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac-Dual_FluA/H7N9

   Plasmid

   #229860

   Purpose

   pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

   Depositor

   Stephen Cusack

   Article

   Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

   Inserts

   PA_H7N9-FluA
   PB1_H7N9-FluA
   PB2_H7N9-FluA
   

   Use

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENC

   Plasmid

   #111163

   Purpose

   miR-E (miR-30 variant)-based RNAi with mCherry reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJP-HBB-nLuc

   Plasmid

   #175431

   Purpose

   Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' HBB leader
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. myristoylated ct-GRK2

   Plasmid

   #178734

   Purpose

   N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

   Depositor

   Ege Kavalali

   Article

   Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

   Insert

   GRK2 (GRK2 Bovine)
   

   Use

   Lentiviral

   Tags

   Src 1-15

   Expression

   Mutation

   only amino acids 548-671

   Promoter

   Ubiquitin

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

    Plasmid

    #122055

    Purpose

    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

    Depositor

    Stephen Tapscott

    Article

    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

    Insert

    MD(ND2bHLH) (Myod1, Neurod2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MyoD chimeric protein substituted with the NeuroD…

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDendra2-H4

    Plasmid

    #157815

    Purpose

    Fluorescent reporter for histone H4 tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H4 (H4c14 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MacroH2A1.1-c002

    Plasmid

    #173087

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N369, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid

    #89587

    Purpose

    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) turboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MacroH2A1.2-c002

    Plasmid

    #173088

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N372, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR LAT-FFF/Zap70 iLID-Drop

    Plasmid

    #171032

    Purpose

    Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

    Plasmid

    #62957

    Purpose

    Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4 in three phases
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-NIrD

    Plasmid

    #65010

    Purpose

    NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

    Depositor

    Wensheng Wei

    Article

    Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

    Inserts

    rtTA-MAVS(C)
    2A-linked delta-TK
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Tight-TRE

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3_altPKA-Cα-mEGFP

    Plasmid

    #114132

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    18747 (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

    Plasmid

    #113549

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDendra2-H3

    Plasmid

    #157814

    Purpose

    Fluorescent reporter for histone H3 tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H3 (H3c1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3xFLAG-CUL7-pCMV7.1

    Plasmid

    #155025

    Purpose

    N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 7 (CUL7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. TERTpWT_Gluc_CCR5

    Plasmid

    #192270

    Purpose

    Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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