We narrowed to 11,551 results for: NSI

1. pB_tetO7_sfGFP

   Plasmid

   #196616

   Purpose

   Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   Superfolder GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   tetO7 promoter

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA_RGG-GFP-RGG

   Plasmid

   #124930

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-ExRai-AktAR

   Plasmid

   #118462

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. UPB-colon (UPB4)

   Plasmid

   #180786

   Purpose

   Unique Projection Barcode (UPB4)-Colon for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial )
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TDP43 NOTAG1

   Plasmid

   #28206

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full length

   Promoter

   Available Since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EYFP-hCaM

   Plasmid

   #47603

   Depositor

   Emanuel Strehler

   Article

   Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

   Insert

   CaM (CALM3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2 GFP-GSK3mut-MAPK

   Plasmid

   #29690

   Purpose

   GFP reporter protein fused to three mutated GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site, GSK3 …

   Promoter

   SP6

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. mEmerald-PDHA1-N-10

    Plasmid

    #54225

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

    Plasmid

    #200123

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-miR-133

    Plasmid

    #172392

    Purpose

    Retroviral expression vector for direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

    Insert

    miR-133
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI SMN1

    Plasmid

    #72286

    Purpose

    SMN1 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    exons 6-7-8 including introns

    Promoter

    CMV

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138129

    Purpose

    Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. a1Ic-HE3-pcDNA3

    Plasmid

    #45810

    Depositor

    Edward Perez-Reyes

    Article

    Gomora et al Biophys J. 2002 Jul;83(1):229-41.

    Insert

    CACNA1I (CACNA1I Human)
    

    Use

    Making crna for oocyte injections

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmGFP-Sec16S

    Plasmid

    #15775

    Depositor

    Benjamin Glick

    Article

    Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

    Insert

    Sec16S (SEC16B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-sapphireCKAR(T/A)

    Plasmid

    #118469

    Purpose

    Negative-control mutant for sapphireCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pARF6(T27N)-CFP

    Plasmid

    #11386

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 27 to Asparagine.

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCer-TG2-YFP

    Plasmid

    #85471

    Purpose

    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

    Depositor

    Jeffrey Keillor, Ray Truant

    Article

    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

    Insert

    hTG2 (TGM2 Human)
    

    Use

    Tags

    EYFP and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGB 35S:YFP:Tnos (GB0209)

    Plasmid

    #68219

    Purpose

    Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

    Plasmid

    #166611

    Purpose

    Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AviTag-SpyTag003-MBP

    Plasmid

    #184227

    Purpose

    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    AviTag-SpyTag003-MBP
    

    Use

    Tags

    AviTag, His6, SpyTag003, and Thrombin cleavage si…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pICH47822_HA-Sr62TK

    Plasmid

    #233535

    Purpose

    Expresses HA-Sr62TK in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK
    

    Use

    Tags

    HA tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICH47822_Sr62NLRa

    Plasmid

    #233537

    Purpose

    Expresses Sr62NLRa in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62NLRa
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. myc-Rictor

    Plasmid

    #1860

    Purpose

    Please see Addgene item #11367 for an updated version of this plasmid.

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWJB9

    Plasmid

    #126650

    Purpose

    Expresses AdrA from a modified pLAFR3

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge