-
-
pCI-neo-cOVA
Plasmid#25097DepositorInsertcytoplasmic ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26
Plasmid#92143PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ABL1
Plasmid#23939DepositorInsertABL1 (ABL1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ eiF4A1 myc HIS
Plasmid#71657Purposemammalian expression of eiF4A1DepositorInserteiF4A1 (EIF4A1 Human)
UseTagsHIS and mycExpressionMammalianMutationaa403 from Ala to ArgPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
7TFP
Plasmid#24308DepositorInsert7xTcf-FFluc // SV40-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Lysosomes-20
Plasmid#54149PurposeLocalization: Lysosome Membrane, Excitation: 487, Emission: 509DepositorInsertLysosomes
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-hSyn-EGFP
Plasmid#114215PurposeLentiviral vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Neo
Plasmid#60509PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCB-hDot1Lwt
Plasmid#74173PurposeFor packaging hDot1Lwt to retrovirus.DepositorInserthDot1L (DOT1L Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGExpressionMutationD10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cep164 (Nigg CW324)
Plasmid#41149DepositorInsertCep164 (CEP164 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOM20-ddGFP-B
Plasmid#40291DepositorInsertTOMM20 (TOMM20 Human)
UseTagsddGFP-BExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1 DN T31N
Plasmid#10833DepositorInsertArf1 T31N (ARF1 Human)
UseTagsHAExpressionMammalianMutationT31N. Defective in GTP binding. Dominant negati…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCM157
Plasmid#45863DepositorInsertcre recombinase
UseCre/LoxTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro
Plasmid#141379PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
WWP-Luc (p21/WAF1 promoter)
Plasmid#16451DepositorInsertWAF1 promoter (CDKN1A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-HDM2
Plasmid#20935DepositorInsertMdm2 p53 binding protein homolog (MDM2 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
A547 (miR-146a promoter)
Plasmid#15091DepositorInsertmiR-146a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mFlagEts1
Plasmid#86099Purposeexpresses flag tagged Ets1 in mammalian cellsDepositorInsertEts1 (Ets1 Mouse)
UseTagsFlag tag; kanamycin selectionExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-FLAG-CD59-GPI
Plasmid#50378PurposeExpress mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorInsertChimsonR-mCherry
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-c-myc-zeo
Plasmid#17758DepositorInsertc-myc (MYC Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-2
UseTags2xSV40NLS-2xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-R1
Plasmid#52638PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertRight AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mMaroon1
Plasmid#83840PurposeExpresses mMaroon1DepositorInsertmMaroon1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F
Plasmid#17438PurposeExpression of UTX in mammalian cellsDepositorInsertUTX (KDM6A Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-N1
Plasmid#54617PurposeLocalization: N1 Cloning Vector, Excitation: 384, Emission: 450DepositorTypeEmpty backboneUseTagsmAzuriteExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSicoR-Ef1a-mCh-Puro
Plasmid#31845PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-L1
Plasmid#52637PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertLeft AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDF0234 pCMV - huDisCas7-11 dead mutant
Plasmid#172509PurposeEncodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cellsDepositorInsertpCMV - huDisCas7-11 dead mutant
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL120-TK
Plasmid#22053DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
CBF:H2B-Venus
Plasmid#44211DepositorInsertCBF1-Responsive-Element
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only
