We narrowed to 14,145 results for: TIM
-
Plasmid#8353DepositorInsertFOXO3a (FOXO3 Human)
TagsHA (YDVPDYASL)ExpressionMammalianMutationT32A, S253A, S315A, mouse estrogen receptor (G525…Available SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFP gRNA (BRDN0000561167)
Plasmid#80034Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cre-responsive Cas9 expressing transposon library vector
Plasmid#164138PurposeBackbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and Synthetic BiologyExpressionMammalianPromoterhuman U6, EF-1aAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-CHMP2A
Plasmid#115328Purposeexpresses mEGFP-CHMP2A in mammalian cellsDepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB1679 CMV-HIVNES-GS-Cas13bt3
Plasmid#176317PurposeCMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)DepositorInserthuman codon optimized Cas13bt3
UseCRISPRTagsHIV NESExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
TagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastPromoterTEFAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
Tags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialPromoterT7Available SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9
Plasmid#169335PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
TagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRRH
Plasmid#136918PurposeExpresses BE4max-NRRH in mammalian cellsDepositorInsertBE4max-NRRH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRTH
Plasmid#136919PurposeExpresses BE4max-NRTH in mammalian cellsDepositorInsertBE4max-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2_mSTING_scrambled_gRNA_2
Plasmid#196628PurposeScrambled control 2 for STING knock-out in murine cells.DepositorInsertmSTING scrambled gRNA 2
UseCRISPR and LentiviralMutationWTAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_2
Plasmid#185017PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_1
Plasmid#185016PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G
Plasmid#216115PurposeEncodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)
Plasmid#134341PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIC3
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM-ER-delta DB
Plasmid#8354DepositorInsertFOXO3a (FOXO3 Human)
TagsHAExpressionMammalianMutationT32A, S253A, S315A, ER (G525R), delta DB (deletio…Available SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-PermE
Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from ermE* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-3xrGBD
Plasmid#176108PurposeVisualization of active RhoDepositorAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-mRFP-pcDNA3.1-
Plasmid#52510Purposeexpresses mRFP in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertmRFP
TagsFLAG-HAExpressionMammalianMutationengineered variant of tetrameric red fluorescent …PromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEF… V…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-VSVG
Plasmid#80606PurposeMammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the proteinDepositorInsertVSV-G
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD40L-MIEG-hCD4 (hCD4-CD40L)
Plasmid#40355DepositorAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …ExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only