Skip to main content
Addgene
Showing: 5361 - 5400 of 8614 results

  3. pFRT-TODestGFP_RNAseH1

    Plasmid
    #65784
    Purpose
    expresses GFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    GFP and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65784-plasmid-map-sequence-id-111845

    Enlarge

  6. pWZL Hygro

    Plasmid
    #18750
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only

  13. pRK7

    Plasmid
    #10883
    Depositor
    John Blenis
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    10883-plasmid-map-sequence-id-6809

    Enlarge

  26. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

    Enlarge

  31. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

    Enlarge

  34. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

    Enlarge

  35. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62203-plasmid-map-sequence-id-467234

    Enlarge

  37. MIGR-RORgt

    Plasmid
    #24069
    Depositor
    Dan Littman
    Insert
    Rorgt (Rorc Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24069-plasmid-map-sequence-id-133147

    Enlarge

Showing: 5361 - 5400 of 8614 results