159,557 results

1. pETDuet S2

   Plasmid

   #171921

   Purpose

   Express RNA-based (p)ppGpp sensor and a NirFP

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Inserts

   RNA-based (p)ppGpp sensor
   NirFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 TFAM-mScarlet

   Plasmid

   #129573

   Purpose

   Expression of mScarlet fused TFAM

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag-TRIM24

   Plasmid

   #28138

   Depositor

   Michelle Barton

   Article

   Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-CAG-rtTA3G-IH

   Plasmid

   #214118

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   trTA3G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

   Plasmid

   #159130

   Purpose

   expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+) mCherry

    Plasmid

    #176301

    Purpose

    Expresses mCherry mRNA from CMV promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-1-BeYDV-LS-L-11

    Plasmid

    #197717

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV LSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-1-BeYDV-LRS-L-11

    Plasmid

    #197718

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV LRSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-mCD47-T2A-GFP

    Plasmid

    #205449

    Purpose

    lentiviral plasmid for expression of mouse CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD47 (Cd47 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-GAS6-COMP5AP-AviTag-9xHis

    Plasmid

    #157511

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    GAS6 (GAS6 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_311-Cas9

    Plasmid

    #96924

    Purpose

    for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

    Depositor

    David Root, William Hahn, John Doench

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFlag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORANGE GFP-Camk2a KI

    Plasmid

    #131484

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6/V5-p53_R280K

    Plasmid

    #22933

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    R280K mutant

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. COVID-SARS2 NSP13

    Plasmid

    #159614

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

    Depositor

    Opher Gileadi

    Article

    Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

    Insert

    Covid-SARS2 Nsp13
    

    Use

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    T7 - LacO

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid

    #191389

    Purpose

    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

    Plasmid

    #159807

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTKEI-Mal-B2

    Plasmid

    #79756

    Purpose

    Single fluorescent protein biosensor for maltose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Mal-B2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMKO.1 puro

    Plasmid

    #8452

    Purpose

    Retroviral vector for shRNA expression.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NRF2

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Myo19

    Plasmid

    #127612

    Purpose

    GFP-tagged Myosin-19

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Myo19
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tet-inducible mCherry reporter

    Plasmid

    #64128

    Purpose

    Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Use

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEGFP-C1-SopF

    Plasmid

    #137734

    Purpose

    Expresses EGFP fused to SopF

    Depositor

    Leigh Knodler

    Insert

    SopF
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Lenti-CMV-PEmax-IRES-ZeoR

    Plasmid

    #207353

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor max (PEmax) enzyme
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEPIP_Peptide_Cloning_Vector

    Plasmid

    #172110

    Purpose

    Lentiviral plasmid for protein / peptide overexpression

    Depositor

    Prashant Mali

    Article

    Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti6/V5-p53_R249S

    Plasmid

    #22935

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R249S Mutant

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge