174,630 results
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pBS mNotch1-IC (DM#270)
Plasmid#15079DepositorAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5 bovine GRK2
Plasmid#14691DepositorInsertGRK2 (GRK2 Bovine)
ExpressionMammalianAvailable SinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-22b DT 51E/148K
Plasmid#11081DepositorInsertDT
TagsHisExpressionBacterialMutation51E/148K, catalytically inactiveAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFR D770-N771 insNPG
Plasmid#11016PurposeRetroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cellsDepositorInsertEGFR D770-N771 insNPG (EGFR Human)
UseRetroviralExpressionMammalianMutationD770-N771 insNPGAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/2
Plasmid#232199PurposeAAV packaging plasmid expressing AAV2 Rep and capsid proteinsDepositorInsertRep2/Cap2
UseAAVExpressionMammalianAvailable SinceSept. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
v5 Cas9-eVLP
Plasmid#228468PurposeExpresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPsDepositorInsertMMLVgag(C507V)–Cas9
TagsFLAGExpressionMammalianMutationMMLVgag(C507V)PromoterCMVAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralPromoterEF1aAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…Available SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorInsertSARS-CoV-2 BA.4/5 Spike (S )
ExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G
Plasmid#177960PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)DepositorInsertSARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
ExpressionMammalianMutationD614GAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
ExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_WT USP7
Plasmid#131242PurposeMammalian expression of N-terminally Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianPromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3M14nes
Plasmid#155367PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertUseCRISPRExpressionMammalianMutationPspCas13b Δ984-1090 H133AAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP14
Plasmid#141267PurposeGateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable SinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3basic luciferase and renilla_polyA
Plasmid#128046PurposepGL3 basic luciferase reporter with co-expression of renilla luciferaseDepositorTypeEmpty backboneExpressionMammalianAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PIP-FUCCI
Plasmid#118621PurposeGateway cloning vector for the cell cycle indicator PIP-FUCCIDepositorInsertPIP-FUCCI
UseGateway CloningTagsmCherry and mVenusAvailable SinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tom20-V5-FKBP-AP_pLX304
Plasmid#120914PurposeLentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membraneDepositorInsertTom20-V5-FKBP-AP
UseLentiviralExpressionMammalianAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
ExpressionMammalianAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable SinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 N-term GFP tTA
Plasmid#107551PurposeAll-in-one dox-repressible expression of cDNA with optional N-term GFP tag.DepositorTypeEmpty backboneUseLentiviralTagsGFP (optional)ExpressionMammalianAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKA-SPARK
Plasmid#106920PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.DepositorInsertPKA-SPARK
ExpressionMammalianAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-SPI1
Plasmid#97039PurposeExpresses SPI1 in mammalian cellsDepositorAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lck-mTurquoise2
Plasmid#98822PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorInsertLck
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (protease)
Plasmid#92214Purposeprotease component of FLAREDepositorInsertCaM-V5-TEVp(Δ220-242)
UseAAVExpressionMammalianPromotersynapsinAvailable SinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Nanoluc
Plasmid#87067PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneTagsNanoluc luciferaseExpressionMammalianAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only
