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We narrowed to 13,087 results for: EGFP

Showing: 6901 - 6950 of 13087 results

  1. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169399-plasmid-map-sequence-id-335422

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  2. pmCellFree_KA1_SARS-COV-2_ORF8

    Plasmid
    #169395
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169395-plasmid-map-sequence-id-336176

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  3. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

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  4. pmCellFree_KA1_SARS-COV-2_N

    Plasmid
    #169401
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _N (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169401-plasmid-map-sequence-id-336866

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  5. pmCellFree_KA1_SARS-COV-2_ORF6

    Plasmid
    #169392
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169392-plasmid-map-sequence-id-335430

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  10. pN256

    Plasmid
    #188472
    Purpose
    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP
    Depositor
    Le Cong
    Article
    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2
    Insert
    eGFP-WPRE-DAISY-4T
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    188472-plasmid-map-sequence-id-370972

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  15. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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  16. L2-AA007

    Plasmid
    #185750
    Purpose
    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185750-plasmid-map-sequence-id-367858

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  17. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85932-plasmid-map-sequence-id-172472

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  18. L2-AA012

    Plasmid
    #185751
    Purpose
    Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185751-plasmid-map-sequence-id-367862

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  21. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

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  23. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  25. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  28. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201955-plasmid-map-sequence-id-405309

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  29. MSCV-Jarid1c-IRES-GFP

    Plasmid
    #166009
    Purpose
    Retroviral biscistronic expression of Jarid1c and eGFP
    Depositor
    Manu Rangachari
    Insert
    Jarid1c (Kdm5c Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166009-plasmid-map-sequence-id-327808

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  30. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

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  31. pKG187

    Plasmid
    #63077
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    63077-plasmid-map-sequence-id-134680

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  32. pIC84

    Plasmid
    #63062
    Purpose
    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63062-plasmid-map-sequence-id-104377

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  33. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

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  35. pIC146

    Plasmid
    #63069
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-P, IMAGE clone 5554572 (CENPP Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63069-plasmid-map-sequence-id-104384

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  45. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

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  46. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  48. pDEST-CMV GFP-LC3B G120A

    Plasmid
    #123115
    Purpose
    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123115-plasmid-map-sequence-id-244026

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  49. Cx32R220stop

    Plasmid
    #78151
    Purpose
    Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA
    Depositor
    Mario Bortolozzi
    Article
    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496
    Insert
    Connexin 32 (GJB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Only contains first 219 residues
    Promoter
    CMV
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 6901 - 6950 of 13087 results