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Addgene

We narrowed to 5,821 results for: ATC

Showing: 521 - 560 of 5821 results
  1. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid
    #73224
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Insert
    PL22-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL22
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pSecEffector

    Plasmid
    #172891
    Purpose
    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
    Depositor
    Inserts
    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ProD, nTETO, and pBAD /AraC
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Insert
    PL31-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL31
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pBW015

    Plasmid
    #131819
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56 with a C-terminal fused mRFP1
    Depositor
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pBW013

    Plasmid
    #131816
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55 with a C-terminal fused mRFP1
    Depositor
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pBW011

    Plasmid
    #131814
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36 with a C-terminal fused mRFP1
    Depositor
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pBW009

    Plasmid
    #131811
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31 with a C-terminal fused mRFP1
    Depositor
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pBW007

    Plasmid
    #131808
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26 with a C-terminal fused mRFP1
    Depositor
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pMP11

    Plasmid
    #215559
    Purpose
    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
    Depositor
    Inserts
    Cas9
    gRNA targeting pBR322 origin of replication
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23105 and tet
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pBL119-2

    Plasmid
    #104336
    Purpose
    Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.
    Depositor
    Insert
    Thiosulfate response regulator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO1
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pCKTRBS-OD

    Plasmid
    #102924
    Purpose
    pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PtetO
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pO3

    Plasmid
    #193748
    Purpose
    Expresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selection
    Depositor
    Insert
    E2-Crimson
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PAAH
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 560 of 5821 results