Skip to main content
Addgene
Showing: 7101 - 7150 of 12238 results

  1. pRD190

    Plasmid
    #163100
    Purpose
    Expression of mEos3.2 in eukaryotes to stain the nuclear compartment
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEos3.2_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163100-plasmid-map-sequence-id-321841

    Enlarge

  3. pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

    Plasmid
    #105207
    Purpose
    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105207-plasmid-map-sequence-id-201286

    Enlarge

  6. p426_Cas9_gRNA-ARS1622b

    Plasmid
    #87403
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87403-plasmid-map-sequence-id-193446

    Enlarge

  9. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121816
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121816-plasmid-map-sequence-id-237364

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

    Enlarge

  17. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

    Enlarge

  23. pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid
    #105192
    Purpose
    Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    105192-plasmid-map-sequence-id-201266

    Enlarge

  24. pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

    Plasmid
    #105209
    Purpose
    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105209-plasmid-map-sequence-id-201289

    Enlarge

  28. pAC8_PH-6His-Nter-GWs-Lox (VE5587)

    Plasmid
    #163768
    Purpose
    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal 6His tag
    Use
    Tags
    6 His Tag
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    163768-plasmid-map-sequence-id-379027

    Enlarge

  29. pAC8_MF-PH-10His-Cter (VE5631)

    Plasmid
    #161802
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10 His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    p10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    161802-plasmid-map-sequence-id-324122

    Enlarge

  30. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid
    #139770
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal TwinStrep tag
    Use
    Tags
    3C - TwinStrep tag
    Expression
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139770-plasmid-map-sequence-id-324179

    Enlarge

  31. pAC8_MF-p10-10His-Cter (VE5742)

    Plasmid
    #139776
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139776-plasmid-map-sequence-id-324157

    Enlarge

  32. pAC8_MF-p10-Flag-Cter(VE5738)

    Plasmid
    #161798
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    p10
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161798-plasmid-map-sequence-id-324175

    Enlarge

  33. pAC8_MF-pH-Flag-Cter (VE5739)

    Plasmid
    #161799
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161799-plasmid-map-sequence-id-324153

    Enlarge

  34. pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)

    Plasmid
    #161800
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal HA tag
    Use
    Tags
    HA
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161800-plasmid-map-sequence-id-324127

    Enlarge

  35. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

    Plasmid
    #161806
    Purpose
    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal 10His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161806-plasmid-map-sequence-id-324125

    Enlarge

  36. pAC8_MF-PH-c-Myc-Cter (VE5632)

    Plasmid
    #139778
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal C-Myc tag
    Use
    Tags
    c-Myc
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139778-plasmid-map-sequence-id-324155

    Enlarge

  37. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid
    #139773
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139773-plasmid-map-sequence-id-324119

    Enlarge

  38. pAC8_MF-PH-HA-Cter (VE5629)

    Plasmid
    #139775
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Hemaglutinine tag
    Use
    Tags
    HA
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139775-plasmid-map-sequence-id-324156

    Enlarge

  39. pAC8_MF-PH-6His-Cter (VE5630)

    Plasmid
    #139777
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 6His tag
    Use
    Tags
    6 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139777-plasmid-map-sequence-id-324174

    Enlarge

  40. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid
    #139769
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    mCherry fluorescent protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    PH or p10
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139769-plasmid-map-sequence-id-324116

    Enlarge

  41. pAC8_MF-PH-3C-10His-Cter (VE5622)

    Plasmid
    #139771
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter 10-His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10-His tag
    Use
    Tags
    3C - 10His
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139771-plasmid-map-sequence-id-324118

    Enlarge

  45. p426_Cas9_gRNA-ARS911b

    Plasmid
    #87394
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87394-plasmid-map-sequence-id-193437

    Enlarge

  49. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid
    #49063
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49063-plasmid-map-sequence-id-72185

    Enlarge

  50. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

    Enlarge

Showing: 7101 - 7150 of 12238 results