We narrowed to 12,555 results for: LIC

1. pLX-B3omega2 (GB1695)

   Plasmid

   #160644

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX-B2alpha2 (GB1696)

   Plasmid

   #160620

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1

   Plasmid

   #46672

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-92a-1 (MIR92A1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CABE-T2.6

   Plasmid

   #193263

   Purpose

   Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.2 mir-1-1 reporter hsa-mir-128-1

   Plasmid

   #46675

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-128-1 (MIR128-1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CBE-T1.6

   Plasmid

   #193275

   Purpose

   Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-MtPylRS (human-opti)

   Plasmid

   #164080

   Purpose

   To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtPylRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEasyG2_mic

   Plasmid

   #184916

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGM55

    Plasmid

    #71249

    Purpose

    luciferase reporter with hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Insert

    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pProEX-Af2Sir2

    Plasmid

    #8380

    Depositor

    Cynthia Wolberger

    Article

    Avalos et al Mol Cell 2002 Sep;10(3):523-35.

    Insert

    Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

13. Delta DED FLIP-L D/A

    Plasmid

    #13791

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Use

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues Asp to Ala m…

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-HA-REDD1 R133A/P139A (#626)

    Plasmid

    #99946

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R133A/P139A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAD-CMV-STAT5A-DNL-CMV-GFP

    Plasmid

    #83253

    Purpose

    Adenoviral expression of STAT5A dominant negative with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5A (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    C-terminal deletion of 351 nucleotides (dominant …

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET11a-Af2sir2

    Plasmid

    #8383

    Depositor

    Cynthia Wolberger

    Article

    Avalos et al Mol Cell 2002 Sep;10(3):523-35.

    Insert

    Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PET11a-Af2Sir2D103N

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPFAS-2XStrep

    Plasmid

    #125682

    Purpose

    expresses human PFAS protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSETa-Amrose v1

    Plasmid

    #79537

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 1

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. peGFPC2-CARKL

    Plasmid

    #51748

    Purpose

    expression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK)

    Depositor

    Markus Ralser

    Article

    Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

    Insert

    SHPK (Shpk Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #79676

    Purpose

    mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L182A/D183A/I184A/G185A catalytic mutant

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pCI-Neo-gp78/2853

    Plasmid

    #11435

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBabe_puro_DEST_Flag_TLOC1 (220aa)

    Plasmid

    #45273

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid

    #172317

    Purpose

    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCarCBAE

    Plasmid

    #117755

    Purpose

    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRII-Sema4a-ISH-2kb

    Plasmid

    #68029

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4A (Sema4a Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    a 2kb cDNA fragment for ISH probe synthesis

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-ERBB4 (E872K)

    Plasmid

    #29535

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed glutamic acid 872 to lysine

    Promoter

    Available since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pLPC-N-Myc-Flag-BirA-hPOT1

    Plasmid

    #166409

    Purpose

    express BirA-hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    BirA, flag, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-ERBB4 (E836K)

    Plasmid

    #29534

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed glutamic acid 836 to lysine

    Promoter

    Available since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1-ERBB4 (E452K)

    Plasmid

    #29529

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed glutamic acid 452 to lysine

    Promoter

    Available since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pQTEV-EPSTI1

    Plasmid

    #34826

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    epithelial stromal interaction 1 (EPSTI1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

    Plasmid

    #166412

    Purpose

    express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 K90E (POT1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    EF-1a

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

    Plasmid

    #121512

    Purpose

    Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgGTTG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPK1E-1-gfp

    Plasmid

    #48133

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-ERBB4 (E542K)

    Plasmid

    #29530

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed glutamic acid 542 to lysine

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    VTC4 (native promoter)

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDM358_DPAKa(GBD)-YFP

    Plasmid

    #128700

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Expression

    Mutation

    none

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mTFP1-Vimentin-7

    Plasmid

    #55515

    Purpose

    Localization: Intermediate Filaments, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

41. MSCV-PM BRD7 sh1

    Plasmid

    #41927

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

42. pcDNA3.1-ERBB4 (E563K)

    Plasmid

    #29532

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed glutamic acid 563 to lysine

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pDONR221-hSOX10-Val15AlafsX11

    Plasmid

    #24732

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    deleted amino acids 44-62. deletion/frameshift…

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

44. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

    Plasmid

    #110735

    Purpose

    Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    H2B-mOrange2
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHBS941 TDP43 RRM-GFP VLIMFYW-S

    Plasmid

    #121992

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobic residues in IDR1&IDR2 mutated…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHBS1383 TDP43 RRM-GFP FYW-S

    Plasmid

    #118791

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All FYW to S in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHBS1386 TDP43 RRM-GFP VLIM-F

    Plasmid

    #118794

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pHBS1387 TDP43 RRM-GFP KRED-S

    Plasmid

    #118795

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All KRED to S in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pPK1E-1+t-gfp

    Plasmid

    #48131

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only