162,536 results

1. pdCas9- AsiA_m2.1

   Plasmid

   #158065

   Purpose

   Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

   Depositor

   Harris Wang

   Article

   Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

   Insert

   dCas9-AsiA_m2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-miniGFP2-P2A-FusionRed

   Plasmid

   #188734

   Purpose

   Express miniGFP2 and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #157027

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   JAG2 (JAG2 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDFDuet-1::ADAT3-ADAT2

   Plasmid

   #15917

   Depositor

   Nina Papavasiliou

   Article

   Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

   Insert

   ADAT3 & ADAT2
   

   Use

   Tags

   FLAG and HexaHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. 7TFC

   Plasmid

   #24307

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-DRGFP

   Plasmid

   #113193

   Purpose

   DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   DR-GFP
   

   Use

   Donor construct with t2a-hygromycin for gene trap…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-IRES-tdTomato-FlagAkt1

   Plasmid

   #64831

   Purpose

   Lentiviral expression of murine Flag-Akt1

   Depositor

   Eva Gonzalez

   Article

   Kajno et al Biochem J. 2015 Apr 9.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJB296

    Plasmid

    #107691

    Purpose

    35SPromoter-LwaCas13a-HSPTerminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP hsp70

    Plasmid

    #15215

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLJM60 FLAG-RAB35 Q67L

    Plasmid

    #107105

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Parkin

    Plasmid

    #213547

    Purpose

    Transient mammalian expresssion of untagged Parkin

    Depositor

    Lani Wu, Steven Altschuler

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pLJM60 FLAG-RAB35 S22N

    Plasmid

    #107106

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Geminin-mStrawberry reporter

    Plasmid

    #158690

    Purpose

    This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG424GPD-ccdB

    Plasmid

    #14152

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65264

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCPRDEnv

    Plasmid

    #1732

    Purpose

    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

    Depositor

    Garry Nolan

    Insert

    Gag-Pol and Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

    Plasmid

    #130996

    Purpose

    Double floxed ChRmine-eYFP under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-Dual-iCas9-CMV

    Plasmid

    #167500

    Purpose

    Expression of dual inducible Cas9 with CMV promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS-mCherry

    Plasmid

    #41583

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p201H Cas9

    Plasmid

    #59176

    Purpose

    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    hph
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLminP_Luc2P_RE17

    Plasmid

    #90359

    Purpose

    NFAT - PKC/Ca++ gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3/Myc-DNMT1

    Plasmid

    #36939

    Purpose

    Mammalian expression of human DNMT1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1(+)IRES GFP

    Plasmid

    #51406

    Purpose

    Expresses GFP from internal ribosome entry sequence, cloning component

    Depositor

    Kathleen_L Collins

    Article

    Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

    Insert

    IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pShuttle

    Plasmid

    #16402

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCEP4-HA-BAX

    Plasmid

    #16587

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    BAX alpha (BAX Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC57-rm_gCarvi-T2A

    Plasmid

    #190334

    Purpose

    Ratiometric reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    ratiometric gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge