We narrowed to 7,988 results for: 104
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Plasmid#56033PurposeLocalization: Nucleus/Centromeres, Excitation: 586, Emission: 632DepositorInsertCENPB (CENPB Human)
TagstdKatushka2ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable SinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-AAV-CAG-iGABASnFR2n-WPRE
Plasmid#218884PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 Exons 1-3
Plasmid#91799PurposeDrosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNADepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2n-6xHis
Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
Tags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLS2-YFPc
Plasmid#87166Purposefluorescence complementation assayDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)
Plasmid#69898PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in mammalian cellsDepositorAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLS2-YFPn
Plasmid#87165Purposefluorescence complementation assayDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH4R-P2A-mCherry-N1
Plasmid#84333PurposeCo-expresses (untagged) Histamine-4 receptor and mCherryDepositorAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2n-WPRE
Plasmid#218872PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVExpressionMammalianMutationS99A F104H R168PPromoterGFAPAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH3R-P2A-mCherry-N1
Plasmid#84332PurposeCo-expresses (untagged) Histamine-3 receptor and mCherryDepositorAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEE062
Plasmid#176819PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert701
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
TagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW683 - pAHP6>>
Plasmid#115998Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD WT
Plasmid#164008PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARDDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRSETC-RdgBbeta-splice variant 1
Plasmid#58276Purposebacterial expression of human RdgBbeta splice variant 1DepositorAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-gfap-sgRNA
Plasmid#65566Purposein vitro trancription of sgRNA targeting the zebrafish gfap locusDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
gp64-mVEGF-C-HisTag-pENTR
Plasmid#64065PurposePlasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.DepositorAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW725 - pUFO>>
Plasmid#116000Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)-NLS
Plasmid#192443PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVL47 - pSCR>>
Plasmid#116013Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0720
Plasmid#141672PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W
Plasmid#105037PurposeLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W
Plasmid#105038PurposeLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 integrase (GB1496)
Plasmid#160578PurposeStreptomyces phage PhiC31 integrase, complete CDSDepositorInsertPhiC31
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVL46 - pWOX4>>
Plasmid#116012Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon
Plasmid#69899PurposeExpresses the human HIPK3 exon 2 circular RNA in mammalian cellsDepositorAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 Catalytic Mutant in pET29b-Strep
Plasmid#48969PurposeExpresses Full length catalytic mutant MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
TagsHis and StrepExpressionBacterialMutationCatalytic Mutant C464APromoterT7Available SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
TagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 P1214R
Plasmid#166822PurposeGateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFRS17A-1
Plasmid#156011PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Blk-SH2
Plasmid#46407DepositorInsertBlk (BLK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 119-224PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-N
Plasmid#27418DepositorInsertZonula Occludens 2 (TJP2 Human)
Tags2xFlagExpressionMammalianMutationDeletion of the carboxy-terminus of ZO-2, so only…Available SinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
HBM-mVEGF-C-HisTag-pENTR
Plasmid#64066PurposePlasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.DepositorAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(L)-AP-His
Plasmid#72013PurposeExpresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pVL44 - pVND7>>
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1 Catalytic Mutant
Plasmid#48971PurposeExpresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
TagsHisExpressionBacterialMutationContains AA 329-566; Catalytic Mutant C464APromoterT7Available SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-ΔCT
Plasmid#64062Purposemyc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationDeletion of C-terminal Asp338–Ser443Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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