We narrowed to 1,595 results for: P11

1. pCMV HA hRB delta CDK + S788

   Plasmid

   #58916

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S788 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCMV HA hRB delta CDK + T356

   Plasmid

   #58911

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T356 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV HA hRB delta CDK + T821

   Plasmid

   #58920

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T821 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV HA hRB delta CDK + T826

   Plasmid

   #58921

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T826 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. 496 pSG5L HA RB del651 (NAAIRS)

   Plasmid

   #10726

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   RB del651 (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 651 to 656 were replaced by NAAIRS, a fl…

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

6. 500 pSG5L HA RB del685 (NAAIRS)

   Plasmid

   #10729

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   RB del685 (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 685 to 690 were replaced by NAAIRS, a fl…

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pCMV HA hRB delta CDK + S249

   Plasmid

   #58908

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S249 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEF1a-RoninDHSA-FLAG-IRES-Neo

   Plasmid

   #28021

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DHSA mutation (Y246A) of the previously defined H…

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RB-409-815-pGex2T

   Plasmid

   #21784

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 409-815 of wt Rb …

   Promoter

   Available since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV HA hRb delta CDK + S230

    Plasmid

    #58907

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S230 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCMV HA hRB delta CDK + T252

    Plasmid

    #58910

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T252 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TFORF1552

    Plasmid

    #144184

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP110 (SP110 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF1555

    Plasmid

    #143825

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP110 (SP110 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1554

    Plasmid

    #143294

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP110 (SP110 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRC/CMV-TYK2VF/PA-VSV

    Plasmid

    #139351

    Purpose

    Expression of the double mutant of TYK2-V678F-P1104A

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F-P1104A

    Promoter

    CMV

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK3-Unstructured-EIF3B-465-552

    Plasmid

    #136029

    Purpose

    EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psiCHECK3-EIF3B-88nt-Delta-Hairpin

    Plasmid

    #136030

    Purpose

    EIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK3-EIF3B-88nt-1st-Hairpin

    Plasmid

    #136031

    Purpose

    EIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psiCHECK3-EIF3B-88nt-Disrupted-Hairpin

    Plasmid

    #136032

    Purpose

    EIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK3-EIF3B-88nt-Restored-Hairpin

    Plasmid

    #136033

    Purpose

    EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. psiCHECK3-EIF3B-88nt-A:U-Hairpin

    Plasmid

    #136034

    Purpose

    EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psiCHECK3-EIF3B-465-552-Unstructured

    Plasmid

    #136028

    Purpose

    EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused downstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

    Plasmid

    #238003

    Purpose

    Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Inserts

    AtRBX1 (RBX1 Mustard Weed)
    AtSKP1 (SKP1 Mustard Weed)
    AtCUL1 (CUL1 Mustard Weed)
    

    Use

    Tags

    S and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMSCV-IRES-GFP/CREB3L1

    Plasmid

    #214767

    Purpose

    Mammalian expression of human CREB3L1

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    CREB3L1 (CREB3L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LentiCRISPR v2 RB1 sgRNA #1

    Plasmid

    #202516

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LentiCRISPR v2 RB1 sgRNA #2

    Plasmid

    #202519

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA 3.2/V5-DEST MTFR1L-V5

    Plasmid

    #69822

    Purpose

    Expresses MTFR1L-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Mitochondrial fission regulator 1-like (MTFR1L Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF0641

    Plasmid

    #143232

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry-3xNLS-OptoJNKi

    Plasmid

    #89740

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKCAG-mCherry-OptoJNKi

    Plasmid

    #89738

    Purpose

    Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmCherry-NES-OptoJNKi

    Plasmid

    #89739

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKCAG-H2BmCherry-OptoJNKi

    Plasmid

    #89741

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Optogenetics

    Tags

    Histone2B and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PIK3CD gRNA (BRDN0001146545)

    Plasmid

    #76165

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PIK3CD gRNA (BRDN0001146527)

    Plasmid

    #76164

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Rb-379-928Δ703-737-pGex2T

    Plasmid

    #21933

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D703-737 o…

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Rb-379-928Δ738-775-pGex2T

    Plasmid

    #21783

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D737-774 o…

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSpCas9 (BB)-2A-GFP-RB1sg

    Plasmid

    #229857

    Purpose

    single guide RNA targeting human RB1 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    RB1sgRNA (RB1 Human)
    

    Use

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

    Plasmid

    #212675

    Purpose

    Express tagged hRb phosphosite mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    15 CDK phospho-sites are mutated to alanines, and…

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 1BR9-AtWUSSTOP-Δα3

    Plasmid

    #202056

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    third helix of homeodomain deleted (AA82-102)

    Promoter

    T7 Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLuc-OptoJNKi

    Plasmid

    #89744

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter and U6

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Rb-379-928-pGex2TK

    Plasmid

    #21749

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCMV HA hRb delta CDK

    Plasmid

    #58906

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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44. pT3EF5a-PIK3CA H1047R

    Plasmid

    #180034

    Purpose

    Entry vector of PIK3CA H1047R for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pTER RB1shRNA human

    Plasmid

    #66886

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

    Insert

    retinoblastoma 1 (RB1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pENTR1A-PIK3CA E545K

    Plasmid

    #180032

    Purpose

    Entry vector of PIK3CA E545K for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAP1892-1

    Plasmid

    #105247

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid

    #89743

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi dark-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid

    #89742

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi lit-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. LT3GEPIR-Rb1shRNA1

    Plasmid

    #220584

    Purpose

    To inducibly knockdown Rb1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    Rb1 shRNA1 (RB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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