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Showing: 7601 - 7650 of 8696 results

  1. MTK0_046

    Plasmid
    #123976
    Purpose
    Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hThal5.10-2_hg18 CAS9 Destination - HygroR
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123976-plasmid-map-sequence-id-251798

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  7. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  8. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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  11. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

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  12. pOCC194

    Plasmid
    #118882
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Tags
    SNAP tag, cleavable with TEV protease, HIS6, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118882-plasmid-map-sequence-id-232359

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  13. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

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  14. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

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  18. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  20. pGGA-FT guide RNA1_7

    Plasmid
    #246797
    Purpose
    A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs
    Depositor
    Danhua Jiang
    Insert
    AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Synthetic, Mustard Weed)
    Use
    CRISPR; Greengate cloning entry vector
    Expression
    Plant
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    246797-plasmid-map-sequence-id-495594

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  22. pREC1010-opEas1

    Plasmid
    #240701
    Purpose
    RSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240701-plasmid-map-sequence-id-489548

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  23. pTARGEX-VAC

    Plasmid
    #242450
    Purpose
    Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    C-terminal propeptide (CTPP) derived from N. taba…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242450-plasmid-map-sequence-id-489544

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  28. pRECBRR1-Kva1

    Plasmid
    #240703
    Purpose
    inverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Kva1 RT,Kva1 ncRNA, PaRecT and PaSSB
    Expression
    Bacterial
    Mutation
    Inverted BRR1 origin of replication
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240703-plasmid-map-sequence-id-483567

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  29. pREC1010C-Eco1

    Plasmid
    #240690
    Purpose
    RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240690-plasmid-map-sequence-id-483599

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  34. pREC1010-opEbu1

    Plasmid
    #240702
    Purpose
    RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240702-plasmid-map-sequence-id-482374

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  35. pREC1010-Abe1

    Plasmid
    #240683
    Purpose
    RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240683-plasmid-map-sequence-id-482346

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  40. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236041-plasmid-map-sequence-id-469952

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  41. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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  45. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  47. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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Showing: 7601 - 7650 of 8696 results