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pEGFP-C2_SEPT9 i4

Plasmid

#71617

Purpose

mammalian expression of human SEPT9 i4 with a GFP fusion

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ265E9

Plasmid

#213472

Purpose

Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadDE-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tadde-32aa linker-zspc…

Tags

NO

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6.ΔBuz-FLAG

Plasmid

#30484

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the C-terminal Ubiquitin binding doma…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-YAP2

Plasmid

#19055

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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Activin A (S2-2)

Plasmid

#184621

Purpose

for transfection into S2 cells and expression of Activin A protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

INHBA (INHBA Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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Control-1

Plasmid

#204442

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L243A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM2

Plasmid

#44239

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M2 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-mApg5

Plasmid

#22958

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Abl-His6-FLAG

Plasmid

#52684

Purpose

expression of Abl in mammalian cells

Depositor

Benjamin Turk

Article

Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

Insert

ABL1 (Abl1 Mouse)

Tags

FLAG and His6

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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hsDCX-pHAT

Plasmid

#83928

Purpose

hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

human Doublecortin isoform b (DCX Human)

Tags

6-His tag and Strep-TagII

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5b-Myc

Plasmid

#186614

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2-hSyn-G-Flamp1-mut

Plasmid

#188570

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

AAV

Tags

His tag

Promoter

hSyn

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Down-Tandem Donor

Plasmid

#229783

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Expression

Mammalian

Promoter

EF1α

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet2 CD

Plasmid

#72219

Purpose

Express flag-tagged mouse Tet2 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET2 catalytic domain.

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pCIG PIK3CA Wildtype

Plasmid

#73056

Purpose

Human PIK3CA Wildtype

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA Wildtype (PIK3CA Human)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-GFP

Plasmid

#220350

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89870

Purpose

expresses DRD2-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS838 TDP43 RRM-GFP

Plasmid

#98249

Purpose

TDP43 RRM-GFP reporter construct

Depositor

Rajat Rohatgi

Article

Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

Insert

TARDBP (TARDBP Human)

Tags

Internal GFP to replace RRM domains

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-dCas13b-ADAR2DD

Plasmid

#190913

Purpose

Addgene #103871 with 3x FLAG on N-terminal of dCas13

Depositor

Timothy Jarome

Article

Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

Insert

3X FLAG

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

Plasmid

#229794

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 deltaN47 Beta-catenin

Plasmid

#19287

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-47

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRST7-ER beta (long)

Plasmid

#11356

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMXs-hu-N-Myc

Plasmid

#50772

Purpose

retroviral expression of human N-Myc

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

N-myc (MYCN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-EPLIN beta

Plasmid

#40929

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
B2345_BTB2x-mCherry

Plasmid

#215097

Purpose

Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB-BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HP35 tension sensor

Plasmid

#101250

Purpose

Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCL-HA-MAPKK1-11A55B [S218E/S222D]

Plasmid

#40809

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

S218E, S222D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HRAS

Plasmid

#55653

Purpose

expression of mouse HRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

HRAS (Hras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

Plasmid

#183150

Purpose

Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Inserts

Lex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD

Use

For yeast genomic integration

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-1-Optimized E6AP

Plasmid

#165100

Purpose

ecoli optimized expression of GST-fusion of human E6AP

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

Insert

E6AP isoform 3 (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SGLT2(GFP)-MAP17(nb)

Plasmid

#216211

Purpose

for expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT2 GFP fusion and MAP17 nanobody fusion (PDZK1IP1, SLC5A2 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
HA-POMP

Plasmid

#86765

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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