We narrowed to 9,393 results for: Coli

1. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOPINS-UBE3C

   Plasmid

   #66711

   Purpose

   Expresses UBE3C HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KiAA0010 (UBE3C )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAC-GAMMAipi

   Plasmid

   #53278

   Purpose

   Has ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   crtYm
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP01

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Parp14-c011

   Plasmid

   #173098

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K791-F978

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tUI-HA

   Plasmid

   #122662

   Purpose

   Express tUI-HA protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tUI-HA
   

   Use

   Tags

   HA-tag and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pPLP1.5

   Plasmid

   #22614

   Depositor

   Lynn Hudson

   Article

   Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

   Insert

   human Proteolipid Protein cDNA (PLP1 Human)
   

   Use

   E. coli phagemid vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pET-MAT2A

   Plasmid

   #164820

   Purpose

   Expresses MAT2A in E. coli BL21(DE3)

   Depositor

   Vern Schramm

   Article

   Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

   Insert

   Methionine Adenosyltransferase 2A (MAT2A Human)
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEVA221rAPOBEC1-T7RNAP

    Plasmid

    #167979

    Purpose

    Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    rAPOBEC1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETSG

    Plasmid

    #159418

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLAREG

    Plasmid

    #73044

    Purpose

    Inducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.

    Depositor

    David McMillen

    Article

    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

    Inserts

    EGFP
    LacI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 promoter and hybrid dual-mode promoter-see c…

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGG-TurboID

    Plasmid

    #209391

    Purpose

    Transiently expressing TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PclpB

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Syt1 C2AB (96-421)

    Plasmid

    #170643

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBSV2H

    Plasmid

    #118229

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMZ3012

    Plasmid

    #204400

    Purpose

    A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

    Inserts

    quorum sensing regulator rhlR (rhlR )
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PftsH and Synthetic promoter PRhl0L0L12

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX Syt1 C2AB (96-421) D303G

    Plasmid

    #170646

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX Syt1 C2AB (96-421) D365E

    Plasmid

    #170649

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX Syt1 C2AB (96-421) I367T

    Plasmid

    #170652

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWaldo-GFPd_PepTSt

    Plasmid

    #58333

    Purpose

    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

    Insert

    PepTSt
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pAC-crRNA-Cm

    Plasmid

    #158711

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    eforCP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMGB

    Plasmid

    #139932

    Purpose

    M. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)

    Depositor

    Christopher Voigt

    Article

    Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23101

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXD69m2-AphA

    Plasmid

    #191247

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET26b_Nb.AT110

    Plasmid

    #135480

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRL1383a

    Plasmid

    #70692

    Purpose

    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMVS1111A:PhmtB-bgaB

    Plasmid

    #205998

    Purpose

    Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

    Depositor

    Bastian Molitor

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Insert

    thermostable beta-galactosidase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PhmtB

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRBBm59

    Plasmid

    #48115

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Atg24 1B

    Plasmid

    #197046

    Purpose

    His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

    Insert

    Atg24
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. VV001: WT Arch-3 in pET28b

    Plasmid

    #58487

    Purpose

    Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3 (with C-terminal His tag)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21a-trAtCCD8(pYL735)

    Plasmid

    #178286

    Purpose

    Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. VV020: WT Arch-3 - eGFP in pET28b

    Plasmid

    #58488

    Purpose

    Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3
    

    Use

    Tags

    eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. His6-Twin-Strep-SUMO-pET28a-EfaCas2

    Plasmid

    #112794

    Purpose

    To express enterococcus faecalis Cas2 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas2
    

    Use

    Tags

    His6-Twin-Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge