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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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258/-2.5lacZ

Plasmid

#25429

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX4-AS-long (pcDNA3.1+)

Plasmid

#75208

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (Slc24a4 Mouse)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pOGG014

Plasmid

#113988

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 4

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTK64_GFP-NuMA-Bonsai

Plasmid

#46351

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA deleted 414-1549 (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acid 414-1549; L1589F mutation in N…

Promoter

pBABE

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSopBWT

Plasmid

#112512

Purpose

PsopB driven sopB and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB/sigE operon

Expression

Bacterial

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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YCe1891 HC_Kan_SV40-ORI_p25

Plasmid

#100679

Purpose

part designed to occupy position 25 of EMMA. Functional category: Viral origin of replication

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40-ORI

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p63(1-29)-mCherry-N1

Plasmid

#67895

Purpose

Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

p63RhoGEF(1-29)-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Tags

V5

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtPT4

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMT-Mad2-GFP

Plasmid

#24284

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Mad2 (mad2 Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
TOPO mKO2

Plasmid

#68442

Purpose

TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKusabira-Orange2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-281&390-428.wt

Plasmid

#12607

Depositor

David McKay

Article

Bitto et al J Biol Chem. 2003 Dec 5. 278(49):49316-2

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF102

Plasmid

#13236

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF102

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pTAL3

Plasmid

#31034

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Tags

AcV5 and FokI nuclease

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1R

Plasmid

#68236

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPyCAG-Myc-ERas-IP

Plasmid

#15690

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Tags

Myc (6x)

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
TAL2368

Plasmid

#36779

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Left (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pNG2

Plasmid

#30986

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG2

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-RFP

Plasmid

#166841

Purpose

Expresses Vac8-RFP in yeasts

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

RFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTAL2

Plasmid

#31033

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALE fusion

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTK52_GFP-NuMA-C-3A

Plasmid

#46350

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115, and changed T…

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 0

Plasmid

#28226

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
BSP602

Plasmid

#122249

Purpose

expression vector for mScarlet under EFT3 Promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mScarlet

Use

Synthetic Biology

Expression

Worm

Promoter

EFT3

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2218

Plasmid

#36677

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MUTYH-TALEN_Left (Mutyh Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGZ12.0501

Plasmid

#141164

Purpose

TcTT2 in Ti plasmid binary vector pGZ12.0501 (KF871320.1)

Depositor

Mark Guiltinan

Article

Florez et al BMC Plant Biol. 2015 May 16;15:121. doi:

Insert

TcTTC

Expression

Plant

Promoter

E12-omega

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-iLifeact-NeonGreen

Plasmid

#175258

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

iLifeact

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGG015

Plasmid

#113989

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 5

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGG008

Plasmid

#113982

Purpose

Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Kanamycin/neomycin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 6201 - 6240 of 17506 results

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We narrowed to 17,506 results for: Emb