Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

167,150 results

Showing: 7801 - 7850 of 167150 results

  1. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Insert
    LDLR (LDLR Human)
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98184-plasmid-map-sequence-id-490370

    Enlarge

  3. TFORF0915

    Plasmid
    #143451
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOA2 (NCOA2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143451-plasmid-map-sequence-id-397908

    Enlarge

  8. mCherry-Calreticulin-N-16

    Plasmid
    #55006
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55006-plasmid-map-sequence-id-470324

    Enlarge

  9. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

    Enlarge

  12. pHIS-GB1-BP1

    Plasmid
    #133427
    Purpose
    Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    SREBF1 (SREBF1 Human)
    Expression
    Bacterial
    Mutation
    SREBP1a amino acids 17-42 fused to GB1 solubilit…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133427-plasmid-map-sequence-id-267726

    Enlarge

  15. pNL [NlucP/CRE/Hygro] Vector

    Plasmid
    #236829
    Purpose
    Express CRE response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236829-plasmid-map-sequence-id-471830

    Enlarge

  17. HCTR1-DuET

    Plasmid
    #213310
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCTR1 (HCRTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213310-plasmid-map-sequence-id-430848

    Enlarge

  18. EYFP-ER-0

    Plasmid
    #56589
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

    Enlarge

  20. pCR4-CTE

    Plasmid
    #36868
    Depositor
    Mario Chin
    Insert
    CTE
    Available Since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36868-plasmid-map-sequence-id-46085

    Enlarge

  22. CMV-ER-LAR-GECO1

    Plasmid
    #61244
    Purpose
    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells
    Depositor
    Robert Campbell
    Article
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Insert
    LAR-GECO1
    Tags
    ER-retention sequence: KDEL and ER-targeting sequ…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1: V51W/I113V/N35…
    Promoter
    CMV
    Available Since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61244-plasmid-map-sequence-id-117077

    Enlarge

  28. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

    Enlarge

  30. pAIDA1

    Plasmid
    #79180
    Purpose
    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags
    Depositor
    Gen Larsson
    Article
    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi
    Insert
    Adhesin Involved in Diffuse Adherence
    Tags
    His and Myc
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    79180-plasmid-map-sequence-id-170854

    Enlarge

  31. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

    Enlarge

  34. pNL [NlucP/CEBP-RE/Hygro] Vector

    Plasmid
    #236832
    Purpose
    Express CEBP response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CEBP (CEBPA Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236832-plasmid-map-sequence-id-471833

    Enlarge

  36. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

    Enlarge

  43. pLV-Golgi eGFP

    Plasmid
    #79809
    Purpose
    To express and target GFP into the Golgi apparatus using lentiviruses
    Depositor
    Pantelis Tsoulfas
    Insert
    Golgi eGFP (B4GALT1 Human)
    Use
    Lentiviral
    Tags
    Fusion of the first 62 amino acids of the human b…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    79809-plasmid-map-sequence-id-150299

    Enlarge

  44. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177434-plasmid-map-sequence-id-348465

    Enlarge

  47. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99248-plasmid-map-sequence-id-191832

    Enlarge

Showing: 7801 - 7850 of 167150 results