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Showing: 7851 - 7900 of 33749 results

  3. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  19. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid
    #216286
    Purpose
    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ⍺-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216286-plasmid-map-sequence-id-433335

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  20. pDEST14-DoubleCatcher-β-Lock

    Plasmid
    #216287
    Purpose
    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher β-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216287-plasmid-map-sequence-id-433337

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  21. pDEST14-DoubleCatcher-γ-Lock

    Plasmid
    #216288
    Purpose
    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher γ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216288-plasmid-map-sequence-id-433338

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  22. pDEST14-DoubleCatcher-δ-Lock

    Plasmid
    #216289
    Purpose
    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher δ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216289-plasmid-map-sequence-id-433340

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  23. pDEST14-DoubleCatcher-ε-Lock

    Plasmid
    #216290
    Purpose
    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ε-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216290-plasmid-map-sequence-id-433359

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  24. pCHW110031

    Plasmid
    #222437
    Purpose
    Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222437-plasmid-map-sequence-id-454095

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  31. pET_BGL1

    Plasmid
    #101910
    Purpose
    Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.
    Depositor
    Steve Shih
    Article
    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7+LacO
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    101910-plasmid-map-sequence-id-197169

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  32. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  33. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Propionate inducible promoter (prp)
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  46. pCHW100002

    Plasmid
    #222440
    Purpose
    Level 0, CDS module (AATG-GCTT) containing rice OsL5.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    OsL5 (Oryza sativa)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222440-plasmid-map-sequence-id-454069

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  47. pRKO

    Plasmid
    #49456
    Purpose
    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
    Depositor
    Marc Gartenberg
    Article
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    loxP-kanMX-loxP cassette
    Use
    Cre/Lox
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49456-plasmid-map-sequence-id-73667

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Showing: 7851 - 7900 of 33749 results