We narrowed to 17,061 results for: Por

1. pJB556

   Plasmid

   #115966

   Purpose

   SGP insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 -98/15 SGP 2xTetApt-STOP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. JW617

   Plasmid

   #64809

   Purpose

   CUC3 expression in yeast

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

   Insert

   CUC3 (CUC3 Mustard Weed)
   

   Tags

   GAL4 DNA binding domain and Myc

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TB:7xSMAD_RE:MiniP

   Plasmid

   #133879

   Purpose

   7 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   7 copies the SMAD DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. JW624

   Plasmid

   #64808

   Purpose

   CUC2 expression in yeast

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

   Insert

   CUC2 (CUC2 Mustard Weed)
   

   Tags

   GAL4 DNA binding domain

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIP-GFP-STX17TMΔC

   Plasmid

   #221737

   Purpose

   Stable expression of GFP-STX17TMΔC in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228 and 227-302

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY017 (pcDNA3.1-hPcCpf1)

   Plasmid

   #69989

   Purpose

   Expresses humanized PcCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPcCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPotef-cdc3CB16-NatR

   Plasmid

   #86465

   Purpose

   Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

   Depositor

   Michael Feldbrügge

   Article

   Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

   Insert

   Cdc3
   

   Use

   Ustilago maydis expression

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   Potef

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHisAlix

   Plasmid

   #42576

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   HIS-TEV

   Expression

   Bacterial

   Mutation

   silent mutation A1617G (numbering in ALIX gene)

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pSLIK_hygro_3xflag_Luciferase

    Plasmid

    #136528

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IP/Venus-mULK1

    Plasmid

    #58743

    Purpose

    Expresses Venus-mULK1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGK401red-hENT1

    Plasmid

    #166028

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    pPGK1

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMRX-IP/Venus-mDFCP1

    Plasmid

    #58742

    Purpose

    Expresses Venus-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pSI-535

    Plasmid

    #131130

    Purpose

    gRNA expression vector for C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    C-to-T reporter activation gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Expression

    Mammalian

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Expression

    Mammalian

    Promoter

    Mouse Rhodopsin

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBICD2-TUG

    Plasmid

    #52873

    Purpose

    retroviral expression of TUG

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Nature. 2003 Oct 16;425(6959):727-33.

    Insert

    TUG (Aspscr1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-CD2 (mouse)

    Expression

    Mammalian

    Promoter

    retrovirus LTR

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

22. plko.1/shMCT4 477

    Plasmid

    #99598

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAL190

    Plasmid

    #22279

    Purpose

    Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCB128

    Plasmid

    #205137

    Purpose

    Expression of mCherry-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    mCherry

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. Tac-GFP

    Plasmid

    #162494

    Purpose

    Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHA-ΔN335-Kin1A

    Plasmid

    #31606

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN335 kinesin-1A (Kif5a Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-335

    Promoter

    CMV

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pDN43

    Plasmid

    #61346

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-biNLS11 and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Tac

    Plasmid

    #162489

    Purpose

    Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMPMA3dPlac-PsopB-GFPLVA

    Plasmid

    #23341

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TXNIP-py

    Plasmid

    #200090

    Purpose

    Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    py motifs mutated (aa331-334 and 375-378)

    Promoter

    CMV

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. pGREAT18

    Plasmid

    #170906

    Purpose

    GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pAtUBQ10

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1099-N-cadherin-AAA-YFP

    Plasmid

    #32237

    Depositor

    Kathleen Green

    Article

    Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

    Insert

    N-cadherin (Cdh2 Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pLexm-gfp-Pept2

    Plasmid

    #167988

    Purpose

    expresses rat PepT2 with a c terminal cleavable GFP-His tag.

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    PepT2 (Slc15a2 Rat)
    

    Tags

    cleavable GFP-his8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMIII-Neo-tdTomato

    Plasmid

    #153424

    Purpose

    Retroviral (MSCV) expression of tdTomato fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    tdTomato
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge