We narrowed to 31,327 results for: uros

1. pNM1325

   Plasmid

   #197144

   Purpose

   CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   SpCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   CAG

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-GFP-IRES-hSNCA (S129D)

   Plasmid

   #185719

   Purpose

   AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ser 129 to Asp

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

   Plasmid

   #185718

   Purpose

   AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ser 129 to Ala

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

   Plasmid

   #185716

   Purpose

   AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Mutation

   deleted AA 95-144

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

   Plasmid

   #105196

   Purpose

   targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

   Depositor

   Peter Mombaerts

   Article

   Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

   Insert

   Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

6. FUS_LC_charge_RtoK

   Plasmid

   #139127

   Purpose

   express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Mutation

   Add K and D to FUS LC so it has the same charge p…

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV-gRNA-Bcan-Ntrk1_4

   Plasmid

   #136413

   Purpose

   Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV-gRNA-Myb-Qk_1

   Plasmid

   #136415

   Purpose

   Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Myb)_U6_sgRNA(Qk)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJet-CMV-CD52-bglpA

    Plasmid

    #89704

    Purpose

    Human CD52 expression plasmid with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD52-bglpA (CD52 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-PIG-N3FLAG-TAF12(50-130)

    Plasmid

    #105595

    Purpose

    retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    truncation fragments containing aa (50-130), and …

    Promoter

    MSCV-LTR

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PRKCG gRNA (BRDN0001147740)

    Plasmid

    #76898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRKCG gRNA (BRDN0001146492)

    Plasmid

    #76899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRKCG gRNA (BRDN0001146495)

    Plasmid

    #76900

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRKAR1B gRNA (BRDN0001146606)

    Plasmid

    #76838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PRKAR1B gRNA (BRDN0001144765)

    Plasmid

    #76839

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PRKAR1B gRNA (BRDN0001147089)

    Plasmid

    #76840

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PANK2 gRNA (BRDN0001162474)

    Plasmid

    #76810

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PANK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PANK2 (PANK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PANK2 gRNA (BRDN0001162362)

    Plasmid

    #76811

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PANK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PANK2 (PANK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PANK2 gRNA (BRDN0001144887)

    Plasmid

    #76812

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PANK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PANK2 (PANK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mGDF8 donor CFP-hDF

    Plasmid

    #72590

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hGDF8 donor CFP-hDF

    Plasmid

    #72591

    Purpose

    Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

    Plasmid

    #111072

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

    Plasmid

    #111267

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.5 AB (361-545, Δ395-511)

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

    Plasmid

    #111460

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346A, C643A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

    Plasmid

    #111461

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346D, C643A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

    Plasmid

    #111462

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

    Plasmid

    #111497

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GABA(A) receptor subunit a1SE

    Plasmid

    #49168

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Stephen Moss, Tija Jacob

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    

    Tags

    myc and pHGFP (pHIuorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-GRAB-HaloDA1.0

    Plasmid

    #234408

    Purpose

    Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 3xFlag-eGFP-Flag-SETX

    Plasmid

    #218838

    Purpose

    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

    Depositor

    Albert La Spada

    Article

    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

    Insert

    Senataxin (SETX Human)
    

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDH-CB-Cdkn1a

    Plasmid

    #228914

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Expression

    Mammalian

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAGIG-CDKN1A

    Plasmid

    #228912

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Ai66(RCRL-tdT) targeting vector

    Plasmid

    #61578

    Purpose

    Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-RSR-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    CAG

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAGGS-FuG-B2

    Plasmid

    #67513

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

    Insert

    Fusion Glycoprotein type B2
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Promoter

    CAGGS

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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45. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-Ef1a-Coff/Fon-mCherry

    Plasmid

    #137134

    Purpose

    Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-Ef1a-Coff/Fon-GCaMP6F

    Plasmid

    #137124

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T

    Plasmid

    #236727

    Purpose

    Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

    Depositor

    Xiangmin Xu

    Article

    Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

    Inserts

    cis-regulatory elements 89763
    iCre(R297T)
    4x6T miRNA targeting cassette
    

    Use

    AAV

    Mutation

    R297T

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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