We narrowed to 12,598 results for: 112
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Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG_BIRC6_BIRmut
Plasmid#197968PurposeMammalian cell expression of 3xFLAG-BIRC6_BIRmutDepositorAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CBE-sgRNA
Plasmid#221137PurposeHelper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with Cas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pb459-mU6-sgRNA-EF1a-PuroR
Plasmid#195507Purposepiggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sitesDepositorInsertPuromycin
UseCRISPR; PiggybacExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CenpB 1-166
Plasmid#108475Purposeexpression of GFP-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
TagsEGFPExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM195A
Plasmid#218125PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of gIIIDepositorInsertsCRBN_CTD
M13 pIII
Tags434cI_RR69Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM194A
Plasmid#218124PurposeHybrid circuit displaying hsCRBN-CTD bait and driving expression of luxABDepositorInsertsCRBN_CTD
luxAB
Tags434cI_RR69Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor-IRES-Blast
Plasmid#222633PurposeRho reporterDepositorInsertRhoB Sensor
UseLentiviralPromoterEF1aAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SuperNovaGreen-T2A-dTomato
Plasmid#193798PurposeExpresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertSuperNova Green
UseAAVTagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRCP
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
ExpressionBacterialAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746
Plasmid#207825PurposeDox-inducible expression of ZNF746DepositorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…Available SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
Plasmid#46835PurposeExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM96
Plasmid#133663PurposeIntegration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM100
Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp160-754*
Plasmid#123212PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(K433E)
Plasmid#42514DepositorInsertPyruvate Kinase M2 Lys433->Glu
UseRetroviralTagsFlagMutationChanged lysine 433 to glutamateAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PP-HaloTag-SYT7alpha
Plasmid#179714PurposeLentiviral expression of mouse Syt7 with N terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD
Plasmid#179720PurposeLentiviral expression of plasma membrane-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXSIP-GPR35-mCherry
Plasmid#204711Purposeexpresses GPR35-mCherry in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GYG
Plasmid#31324DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
JAB3312
Plasmid#190406PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
ExpressionPlantAvailable SinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3494
Plasmid#190418PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter
ExpressionPlantAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2602
Plasmid#190389PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
ExpressionPlantAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.4.1
Plasmid#97363PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Flag-RIPK1-wt
Plasmid#102677Purposeretroviral expression plasmid for RIPK1-wtDepositorInsertRIPK1 (RIPK1 Human)
UseRetroviralTagsFLAG and IRES-GFPExpressionMammalianPromoterunknownAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_Luciferase
Plasmid#136528PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No3
Plasmid#194898PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.3
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No9
Plasmid#194903PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.9
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.1
Plasmid#97356PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pACYC-Cas12g1-noncoding
Plasmid#120880PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12g1
UseCRISPRExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2
Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…Available SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-TTF-1-HDD
Plasmid#52063PurposeIt encodes a mutant of TTF-1 in which the homeodomain is deletedDepositorInsertThyroid Transcription Factor 1 (NKX2-1 Human)
ExpressionMammalianMutationHomeodomain deletedAvailable SinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF169
Plasmid#99392PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169DepositorInsertRNF169 (RNF169 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECFP-Rac1Y40C
Plasmid#105293PurposeControl for FRET biosensor analysis - cannot bind downstream effectorsDepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNA500
Plasmid#149583Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mNeptune2
Plasmid#104012PurposePlasmid encoding mNeptune2DepositorInsertmNeptune2
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… R32…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP5.1.1
Plasmid#98271PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf S338A
Plasmid#118335PurposeExpression of human CrafDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
UKNP4.1.1
Plasmid#98266PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only