162,160 results

1. pGEX Cis1-SH2

   Plasmid

   #46417

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Cis1 (SOCS1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 90-214

   Promoter

   Tac

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-ALK

   Plasmid

   #116712

   Purpose

   Lentiviral expression of ALK

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ALK (ALK Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOTv7-blast-nanoluc

   Plasmid

   #179797

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   nanoluc
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-CLASP2 512-650

   Plasmid

   #24409

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (512-650) (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant with only MT plus end trac…

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV hSyn-fDIO-taCasp3-TEVp

   Plasmid

   #207997

   Purpose

   Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

   Depositor

   Kazunari Miyamichi

   Article

   Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

   Insert

   taCasp3-TEVp
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-GcAMP5

   Plasmid

   #126569

   Purpose

   Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-CMV-N-mCherry

   Plasmid

   #123215

   Purpose

   N-terminal mCherry tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMTTH+bromo5

   Plasmid

   #157803

   Purpose

   Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   bromo5
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103 IQGAP1 WW domain

    Plasmid

    #104279

    Purpose

    Bacterial expression of WW domain from IQGAP1

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    IQGAP1 WW domain (IQGAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Socs3-SH2

    Plasmid

    #46506

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs3 (SOCS3 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 22-142

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179430

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. cmv-sfGFP-GB1-scAB

    Plasmid

    #185794

    Purpose

    scFv GFP compnent for SunRISER

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    sfGFP-GB1-scAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 763 pME mApple

    Plasmid

    #195960

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX Socs1-SH2

    Plasmid

    #46504

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-166

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FoxO1-ADA-GFP

    Plasmid

    #35640

    Depositor

    Domenico Accili

    Article

    Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

    Insert

    FoxO1-ADA-GFP (Foxo1 Mouse)
    

    Use

    Tags

    Flag, GFP, and Myc

    Expression

    Mammalian

    Mutation

    T24A, S253D and S316A mutations

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEZYHb

    Plasmid

    #18669

    Purpose

    pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    hemoglobin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pdCRISPomyces-2BD

    Plasmid

    #234429

    Purpose

    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified dCas9(D10A, H840A) with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Flag-FOXK2

    Plasmid

    #153133

    Purpose

    Expression of Flag tagged human FOXK2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXK2 (FOXK2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSmartBAC-S

    Plasmid

    #107268

    Purpose

    Broad host range bacterial artificial chromosome vector

    Depositor

    Mark Liles, Ron Godiska

    Article

    Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SPOP-V5 (BACK))

    Plasmid

    #169722

    Purpose

    Mammalian Expression of mouse SPOP Y353E

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Y353E

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SPOP-V5 (BTB)

    Plasmid

    #169721

    Purpose

    Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPTn2P-PTK

    Plasmid

    #85604

    Purpose

    Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Scott Fahrenkrug, Karl Clark

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Passport transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEW8

    Plasmid

    #84238

    Purpose

    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

    Depositor

    David Weinberg

    Article

    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

    Insert

    HA-mRuby-P2A-eGFP
    

    Use

    Tags

    HA-mRuby and eGFP

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. xGoosecoid

    Plasmid

    #24977

    Depositor

    Edward De Robertis

    Article

    Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

    Insert

    Goosecoid (gsc-a Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

33. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. XP12 Xbra

    Plasmid

    #16978

    Depositor

    Randall Moon

    Insert

    Xbra (t.S Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

35. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBHM2617

    Plasmid

    #234496

    Purpose

    NATR-CnU6-empty sgRNA

    Depositor

    Hiten Madhani

    Article

    Nalley et al bioRxiv 2025.02.14.638277

    Insert

    NAT-PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSP1D1-Strep-II

    Plasmid

    #224209

    Purpose

    Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

    Depositor

    Anne Robinson

    Article

    Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

    Insert

    MSP1D1-Strep-II
    

    Use

    Tags

    Strep-II Tag

    Expression

    Bacterial

    Mutation

    Substitution of 7xHis tag found in pMSP1D1 for a …

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5-FRT/TO-APT2-LaG16-3W

    Plasmid

    #234425

    Purpose

    Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge