19,210 results

1. pcDNA5/FRT/TO-GAlphai1-RLuc8

   Plasmid

   #140973

   Purpose

   Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai1-RLuc8 (GNAI1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV HA hRB-wt

   Plasmid

   #58905

   Purpose

   mammalian expression of wild type human Rb

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Hygro(+)-mscGAS

   Plasmid

   #102607

   Purpose

   Mammalian expression of mouse cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (Cgas Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIE1

   Plasmid

   #136355

   Purpose

   Selection by Inducible Effectors

   Depositor

   Andrew Goodman

   Article

   Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

   Inserts

   Type VI effector bfe1
   Type VI effector bte1
   BT_4676
   tetR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   ATG removed to add signal sequence before gene

   Promoter

   P1TDP-GHO23

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-MHCK7-GCaMP6

   Plasmid

   #65042

   Purpose

   Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

   Depositor

   Nenad Bursac

   Article

   Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

   Insert

   GCaMP6
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   MHCK7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH376

   Plasmid

   #86842

   Purpose

   mamalian expression of anti-CRISPR protein AcrIIA4

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid

    #140005

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pARF1(T31N)-CFP

    Plasmid

    #11384

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 31 to Asparagine.

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH2B-Electra2

    Plasmid

    #179479

    Purpose

    Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPC1-hVAP-B

    Plasmid

    #104448

    Purpose

    Expression of human VAP-B fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and linked by IRES

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Rab7A

    Plasmid

    #28047

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. HA-GABARAPL1

    Plasmid

    #137759

    Purpose

    Expression of GABARAPL1

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GABARAPL1 (GABARAPL1 Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDisplay-pHuji

    Plasmid

    #61556

    Purpose

    Red fluorescent protein based pH indicator in pDisplay vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JD639

    Plasmid

    #160398

    Purpose

    Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PCV-Cas9

    Plasmid

    #123643

    Purpose

    Expresses Cas9 with an N-terminal HUH-tag called PCV2

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Use

    Tags

    (EAAAK)8 linker and His6 + SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJ3M-Mst2

    Plasmid

    #12205

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-NRAS-Q61K

    Plasmid

    #116444

    Purpose

    Lentiviral expression of NRAS Q61K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q61K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge