We narrowed to 12,964 results for: BASE
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Plasmid#169454PurposepRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassetteDepositorInsertgRNA for MATα
ExpressionYeastPromoterSNR52Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-BSR-GFP11
Plasmid#68716PurposeSplit GFP assayDepositorInsertbsr
UseRetroviralTagsFLAG and GFP11ExpressionMammalianAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
Tagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-GOLGA2
Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas-hHDAC3-R265P
Plasmid#103786PurposeExpresses dCas9 fused to human HDAC3 with R265P mutationDepositorInsertdCas9-HDAC3-R265P (HDAC3 S. pyogenes, Synthetic, Human)
UseCRISPR and LentiviralExpressionMammalianMutationChanged arginine 265 to prolinePromoterEF1AAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-luc2
Plasmid#173006Purposefirefly luciferase reporter vector with 10x clustered UAS binding sitesDepositorInsert10xUAS-luc2
UseLuciferaseExpressionMammalianAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-6xHis
Plasmid#62374PurposeExpression of Cas9-6xHis in bacterial cellsDepositorInsertCas9_6xHis
Tags6xHis tagExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
ND6-DdCBE-left side TALE
Plasmid#157842Purposeexpresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p53-TRE-mStrawberry reporter
Plasmid#158684PurposeThis plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1397 DddAtox C
Plasmid#158097Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1397 DddAtox N
Plasmid#158094Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1333 DddAtox C
Plasmid#158095Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1333 DddAtox N
Plasmid#158093Purposeexpresses the left-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI
ExpressionMammalianMutationWTAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p38-KTR-mStrawberry reporter
Plasmid#158687PurposeThis plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAR-TRE-mStrawberry reporter
Plasmid#158683PurposeThis plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-LAMP1
Plasmid#207787PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
JNK-KTR-mStrawberry reporter
Plasmid#158689PurposeThis plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-Y-pyrG
Plasmid#221131PurposeExpression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …PromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ADAR1 p150
Plasmid#237855PurposeLentiviral expression of ADAR1 p150DepositorAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-GFE3
Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorHas ServiceAAV1InsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ADAR1 p110
Plasmid#237854PurposeLentiviral vector expressing ADAR1 p110DepositorAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dCas9
Plasmid#112195PurposeKRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertKRAB (ZNF10 Human)
UseCRISPRTags3XFLag-NLS-KRAB-dCas9-NLSExpressionMammalianPromoterCMVAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)
Plasmid#105200PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-P2A-antiPD1(LC)
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-LMNB1
Plasmid#207770PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-GFP
Plasmid#58245PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFPDepositorInsertsattR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP
UseLentiviral and RNAi; Gateway destionation vectorTagsGFP, NLS, T2A, and TetRExpressionMammalianPromoterSFFV and noAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PKA-KTR-mStrawberry reporter
Plasmid#158688PurposeThis plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-SBP-GFP
Plasmid#120172PurposeExpresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)DepositorInsertLysosomal-associated membrane protein 1 (Lamp1 Rat)
TagsSBP and eGFPExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-VP64-dCas9-VP64
Plasmid#232179PurposeLentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cellsDepositorInsertVP64-dCas9-VP64
UseLentiviralExpressionMammalianMutationD10A, H840APromoterhUbCAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV -HA-Luciferase
Plasmid#227802Purposeexpressing HA tag and the coding sequence of luciferase by the constitutive CMV promoterDepositorInsertHA-Luciferase
UseAAVPromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852Available SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-NbmiR482aTS-B/c
Plasmid#227967PurposeExpression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.DepositorInsertsB/c
NbmiR482aTS
ExpressionPlantPromoter35S and NoneAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H332
Plasmid#228250PurposemT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQ79
Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
ExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin
Plasmid#133028PurposeExpresses EGFP-tagged Golgi apparatus markerDepositorInsertGolgin84 (GOLGA5 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-673PromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …PromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’-LMNA-eGFP-KI
Plasmid#170545PurposeExpresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNADepositorInsertsLeft Homology arm for LMNA knockin
Right Homology arm for VIM knockin
UseCRISPR and LentiviralAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only