165,786 results

1. pFUW LAMP1Sig-mCherry

   Plasmid

   #239556

   Purpose

   Expression and lysosomal localization of mCherry

   Depositor

   Tim Ahfeldt, Joel Blanchard

   Article

   et al iScience 28 (2025)

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   UbC

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUW PuroR-P2A-COX8ASig-Timer

   Plasmid

   #239559

   Purpose

   Expression and mitohcondrial localization of Timer

   Depositor

   Tim Ahfeldt, Joel Blanchard

   Article

   et al iScience 28 (2025)

   Insert

   Timer
   

   Use

   Lentiviral

   Promoter

   UbC

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TurboID-TM

   Plasmid

   #231498

   Purpose

   Expressing TurboID on the cell surface of mammalian cells

   Depositor

   Baljit Khakh

   Article

   Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

   Insert

   TurboID
   

   Tags

   Igk, HA, Myc, PDGFR-beta transmembrane domain

   Expression

   Mammalian

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYO1280

   Plasmid

   #235712

   Purpose

   Expression of FL human VPS34-EGFP in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   VPS34 (PIK3C3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-T-PAF-Clu

   Plasmid

   #215380

   Purpose

   High expression of Clusterin for purification.

   Depositor

   Franz-Ulrich Hartl

   Insert

   CLU (CLU Human)
   

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYFJ84

   Plasmid

   #227644

   Purpose

   Vibrio harveyi acyl-ACP synthetase

   Depositor

   John Cronan

   Article

   Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

   Insert

   Acyl-Acyl carrier protein synthetase
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmEGFP-N1

   Plasmid

   #217486

   Purpose

   Expression of monomeric Enhanced Green Fluorescent Protein

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   mEGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-Leup

   Plasmid

   #213154

   Purpose

   Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

   Depositor

   Jason Crawford

   Article

   Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

   Insert

   Leupeptin Protease Inhibitor Pathway
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid

    #210767

    Purpose

    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    DRSR 514-517 to AS; 557 R to A; multiple silent m…

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBiT3.1-N_RBBP4:1-425

    Plasmid

    #211037

    Purpose

    Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RBBP4:M1-S425 (RBBP4 Human)
    

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE80l BCB

    Plasmid

    #196472

    Purpose

    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    BCB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

    Plasmid

    #175256

    Purpose

    SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-kanMX6-P3nmt1-mECitrine

    Plasmid

    #105151

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    kanMX6-P3nmt1-mECitrine
    

    Tags

    3nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    3nmt1

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-kanMX6-P81nmt1-mECitrine

    Plasmid

    #105153

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

    Insert

    kanMX6-P81nmt1-mECitrine
    

    Tags

    81nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    81nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-kanMX6-P41nmt1-mECitrine

    Plasmid

    #105152

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    kanMX6-P41nmt1-mECitrine
    

    Tags

    41nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    41nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. jk68

    Plasmid

    #87020

    Purpose

    genomic Sindbis virus plasmid for use in SYNseq PLA screen

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    SNAP-Nlg1AB-4xλN(i)
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Expression

    Mammalian

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pH2B_mCherry_IRES_neo3

    Plasmid

    #21044

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. GABA(A) receptor subunit a1SE

    Plasmid

    #49168

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    

    Tags

    myc and pHGFP (pHIuorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-AXL-V5/HIS

    Plasmid

    #200117

    Purpose

    Expression of human AXL receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human AXL receptor tyrosine kinase (AXL Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-Golgi eGFP

    Plasmid

    #79809

    Purpose

    To express and target GFP into the Golgi apparatus using lentiviruses

    Depositor

    Pantelis Tsoulfas

    Insert

    Golgi eGFP (B4GALT1 Human)
    

    Use

    Lentiviral

    Tags

    Fusion of the first 62 amino acids of the human b…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Promoter

    minP

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pICH47742::p35S-ePEmax3::tEURb7

    Plasmid

    #227692

    Purpose

    Golden Gate level 1, position 2, ePEmax3 expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. kiCAP-AAV-BI-hTFR1

    Plasmid

    #218796

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    AAV Rep-Cap BI-hTFR1
    

    Expression

    Mammalian

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge