163,606 results

1. dPspCas13b-mutFTO

   Plasmid

   #223697

   Purpose

   Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   dPspCas13b-GGS-mutFTO (FTO Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC1-4k-BBclone2

   Plasmid

   #137070

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0323
   P13
   DipA
   Lmp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Promoter

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-N1-LATS2

   Plasmid

   #166474

   Purpose

   Expresses fusion of mEGFP and LATS2

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, LATS2 (LATS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1P-iCLHN

   Plasmid

   #66579

   Purpose

   AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

   Plasmid

   #176278

   Purpose

   Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p21 promoter A

   Plasmid

   #16462

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 promoter (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-EF1a ANXA11-mEmerald D40G

   Plasmid

   #164211

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   D40G

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4b Q61H

    Plasmid

    #83140

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61H

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM5057

    Plasmid

    #120085

    Purpose

    Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

    Insert

    RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_IRES_tdTomato

    Plasmid

    #184046

    Purpose

    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

    Depositor

    Angela Pannier

    Article

    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

    Inserts

    Enhanced Green Fluorescent Protein
    tdTomato
    Internal Ribosome Entry Site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCP-APEX2

    Plasmid

    #154936

    Purpose

    NLS-V5-MCP-APEX2

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    NLS-V5-MCP-APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Tags

    Expression

    Mutation

    Promoter

    C. glabrata MET3

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18-mini-Tn7T-Gm-lacZ

    Plasmid

    #63120

    Purpose

    mini-Tn7 lacZ transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA3.1(+)-OTI-TCRA

    Plasmid

    #131035

    Purpose

    Expresses OT-I TCRA

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LXRE_Luc

    Plasmid

    #177622

    Purpose

    synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

    Depositor

    Thomas Burris

    Article

    Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

    Insert

    3X LXRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Use

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-MBP-His-SFP

    Plasmid

    #141141

    Purpose

    Expression of 4'-phosphopantetheinyl transferase gene

    Depositor

    New England Biolabs

    Insert

    4'-phosphopantetheinyl transferase
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. Hs.KRAS4b G12V

    Plasmid

    #83132

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pN256

    Plasmid

    #188472

    Purpose

    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    eGFP-WPRE-DAISY-4T
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. IDG_CACNB2_OE_1

    Plasmid

    #161684

    Purpose

    Overexpresses CACNB2

    Depositor

    Mike McManus

    Insert

    CACNB2 (CACNB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Alpha1

    Plasmid

    #118044

    Purpose

    GoldenBraid2.0 Alpha 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+)-OTI-TCRB

    Plasmid

    #131036

    Purpose

    Expresses OT-I TCRB

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEX-CMV-SP-STIM2(15-746)

    Plasmid

    #18868

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    STIM2 (STIM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Signal peptide(SP) 1-10 from STIM1 and residues 1…

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid

    #218509

    Purpose

    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    mKate2
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Deletion of Plac_mrfp cassette, introduction of a…

    Promoter

    P23

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA5 FRT TO FLAG LRRK1

    Plasmid

    #232062

    Purpose

    Expresses FLAG-LRRK1 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge