8,883 results

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pGL2-Mcl-1

Plasmid

#19132

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5 Smad2-HA

Plasmid

#14930

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-PATagRFP

Plasmid

#31945

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

His

Expression

Bacterial

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

araB

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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Mfn2(K109A)-16xmyc

Plasmid

#26051

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

16xMyc

Expression

Mammalian

Mutation

K109A GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pARF6-YFP

Plasmid

#11389

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 R465C

Plasmid

#16653

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 R465C (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

R465C

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pN1-Lck-GCaMP3

Plasmid

#26974

Depositor

Baljit Khakh

Article

Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

Insert

Lck-GCaMP3

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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aMHC-puro rex-neo

Plasmid

#21230

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCM1.36

Plasmid

#17249

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

tbb-2 3'UTR in pDONRP2RP3

Use

Gateway

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-HA-YOD1

Plasmid

#22554

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

YOD1 (YOD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLHCX-Flag-mPKM2

Plasmid

#42512

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_NDG

Plasmid

#26820

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-NDGFP-3'UTR

Expression

Mammalian

Mutation

nuclear destabilized eGFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
iRFP-FRB-Rab7

Plasmid

#51613

Purpose

Recruiter to Rab7-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab7

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSTIM1 myc

Plasmid

#17732

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-Pax6

Plasmid

#32932

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Pax6 (isoform 2, transcript variant 5) (Pax6 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-TYRO3

Plasmid

#23916

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TYRO3 (TYRO3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pEX-SP-YFP-STIM1(D76A)

Plasmid

#18859

Depositor

Tobias Meyer

Article

Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

D76A mutation - changed aspartate 76 to alanine. …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-IKBalpha-wt

Plasmid

#15290

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-p300 reporter

Plasmid

#11726

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

p300 promoter and 5' UTR (EP300 )

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64219

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MT-cdc20

Plasmid

#11593

Depositor

Marc Kirschner

Article

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdc20 (CDC20 Human)

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pLL3.7_hsa-miR-34a

Plasmid

#25791

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a (TP53 Human)

Use

Lentiviral and RNAi

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetrosuper Fbw7 shRNA-1

Plasmid

#15660

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Fbw7 shRNA (FBXW7 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pESC-LEU-GFP-VHL

Plasmid

#21053

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-NKX6.1

Plasmid

#32934

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

NKX6.1 (NKX6-1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1285

Plasmid

#61252

Purpose

CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting pha-1

Use

CRISPR

Expression

Worm

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MLL2 653

Plasmid

#11017

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MLL2 (KMT2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

truncation of MLL2, last 653 amino acids, from pr…

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
p6592 MSCV-IP N-HAonly WWP2

Plasmid

#34905

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WWP2 (WWP2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag RPS6KB1

Plasmid

#20624

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PGKneobpA

Plasmid

#13442

Depositor

Philippe Soriano

Article

Soriano et al J Virol. 1991 May . 65(5):2314-9.

Insert

PGKneobpA

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-GFP-NMHC II-A

Plasmid

#10844

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
896 pcDNA3 T7 Akt1

Plasmid

#9003

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

Plasmid

#47550

Purpose

Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Inserts

Cas9
ttTi5605 sgRNA

Use

CRISPR

Tags

HA and NLS

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Promoter

U6 and eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Stat4 pRc/CMV

Plasmid

#8711

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat4 (Stat4 Mouse)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-lox-hOCT4

Plasmid

#20728

Depositor

Rudolf Jaenisch

Article

Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1 puro Rb shRNA

Plasmid

#10670

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Rb shRNA (RB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge

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