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170,825 results

Showing: 6721 - 6760 of 170825 results

  2. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Expression
    Mammalian
    Available Since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  3. pCW57.1_SPTLC1_WT

    Plasmid
    #181920
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181920-plasmid-map-sequence-id-361674

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  4. Mito-B

    Plasmid
    #209864
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209864-plasmid-map-sequence-id-415402

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  5. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

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  6. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

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  15. FLT3-ITD-MSCV-IRES-GFP

    Plasmid
    #184779
    Purpose
    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Activating internal tandem duplication (ITD) adde…
    Promoter
    MSCV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184779-plasmid-map-sequence-id-364900

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  17. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Vsevolod Belousov
    Insert
    mito-DAAO
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    141132-plasmid-map-sequence-id-323598

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  21. pTFE3.1.0-gDNA

    Plasmid
    #112473
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3
    Depositor
    Kevin White
    Insert
    TFE3 (TFE3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112473-plasmid-map-sequence-id-230504

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  28. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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Showing: 6721 - 6760 of 170825 results