Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 16,979 results for: She

Showing: 8651 - 8700 of 16979 results

  1. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

    Enlarge

  3. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid
    #90241
    Purpose
    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90241-plasmid-map-sequence-id-178296

    Enlarge

  4. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

    Enlarge

  5. PTPN11

    Plasmid
    #38965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN11 (PTPN11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pLKO-Tet-On-HDAC1-shRNA1

    Plasmid
    #90242
    Purpose
    Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90242-plasmid-map-sequence-id-178298

    Enlarge

  7. Hs.KRAS4a G12V

    Plasmid
    #83169
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83169-plasmid-map-sequence-id-158132

    Enlarge

  10. pLEX305_3xFLAG_RELA1

    Plasmid
    #220324
    Purpose
    Expresses full length human RELA with an N-terminal 3xFLAG tag
    Depositor
    Jonathan Brown
    Insert
    RELA (RELA Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220324-plasmid-map-sequence-id-437247

    Enlarge

  15. pSA120

    Plasmid
    #59789
    Purpose
    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59789-plasmid-map-sequence-id-95470

    Enlarge

  17. Hs.KRAS4a G12C

    Plasmid
    #83167
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12C
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83167-plasmid-map-sequence-id-158130

    Enlarge

  19. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

    Plasmid
    #75295
    Purpose
    An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.
    Depositor
    Brandon Harvey
    Insert
    eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    human SYN1
    Available since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75295-plasmid-map-sequence-id-142411

    Enlarge

  21. BRAF - A12G in pENTR

    Plasmid
    #216606
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Douglas Allan, Christopher Loewen
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    A12G
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216606-plasmid-map-sequence-id-427164

    Enlarge

  23. Lamin A (R527P)-mRFP

    Plasmid
    #124272
    Purpose
    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R527P) (LMNA Human)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R527P)
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124272-plasmid-map-sequence-id-241977

    Enlarge

  24. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

    Enlarge

  25. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

    Enlarge

  28. Lamin A (R482Q)-mRFP

    Plasmid
    #124273
    Purpose
    Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R482Q) (LMNA Human)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R482Q)
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124273-plasmid-map-sequence-id-241964

    Enlarge

  29. HSP90B1

    Plasmid
    #39076
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSP90B1 (HSP90B1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid
    #194330
    Purpose
    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein
    Depositor
    Alexander McLellan
    Insert
    Her2-Fc (ERBB2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194330-plasmid-map-sequence-id-383149

    Enlarge

  32. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pACP-ADRβ2 Control Plasmid

    Plasmid
    #101128
    Purpose
    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Beta-2-Adrenergic Receptor (ADRB2 Human)
    Use
    Tags
    ACP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101128-plasmid-map-sequence-id-194168

    Enlarge

  37. HSD17B11

    Plasmid
    #39159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B11 (HSD17B11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. Lamin A (R298C)-mRFP

    Plasmid
    #124269
    Purpose
    Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R298C) (LMNA Human)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R298C)
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124269-plasmid-map-sequence-id-241969

    Enlarge

  40. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid
    #85398
    Purpose
    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    S27A
    Promoter
    human Synapsin1 promotor and ribosomal skip seque…
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    85398-plasmid-map-sequence-id-201308

    Enlarge

  41. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

    Enlarge

  44. huntingtin_V2095-V3138_CbioCflag

    Plasmid
    #194764
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194764-plasmid-map-sequence-id-402574

    Enlarge

  45. huntingtin_T97-M2069_NbioCflag

    Plasmid
    #194763
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194763-plasmid-map-sequence-id-402570

    Enlarge

  46. Hs.KRAS4a G13D

    Plasmid
    #83170
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G13D
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83170-plasmid-map-sequence-id-158133

    Enlarge

  50. pDONR221_SMAD2_HAe2_Bsd

    Plasmid
    #167166
    Purpose
    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
    Depositor
    Alexander Loewer
    Insert
    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    Use
    Repair template for gene editing in human cells
    Tags
    blasticidin
    Expression
    Mutation
    Promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167166-plasmid-map-sequence-id-330264

    Enlarge

Showing: 8651 - 8700 of 16979 results