We narrowed to 13,226 results for: 109
-
-
pColE1_sgRNA_M4NS_AmpR
Plasmid#119156PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-RPL23a-C1
Plasmid#134535PurposeExpresses YFP-RPL23a fusion proteinDepositorInsertRPL23a (RPL23A Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PBDGTV
Plasmid#100859PurposeA piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc2-tetA
Plasmid#166982PurposeGenerates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertCspRecT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpVanCCAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin G76C
Plasmid#86583PurposeBacterial expression of Ubiquitin G76C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-L1PA8CHmneo
Plasmid#69608PurposeExpresses a neomycin tagged codon optimized L1PA8DepositorInsertL1PA8
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMP4516
Plasmid#107775PurposeExpression of ECFPDepositorInsertpLacZ-ECFP in pBBR1-mcs5
UseTagspLacZ-ECFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/WT myc-M1
Plasmid#87719PurposeExpresses WT myc-M1 spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM1 isoformPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_AH
Plasmid#148918PurposeInsect Expression of DmGIGYFDepositorInsertDmGIGYF (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1.530
Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-mPlum
Plasmid#158062PurposeDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertmonomeric plum fluoresecent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM115: CasRx control presgRNA
Plasmid#166867PurposeU6-driven expression of control (non-targeting) presgRNA compatible with CasRx.DepositorInsertnon-targeting presgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Suv39H1_2
Plasmid#36343DepositorInsertSuv39H1 (SUV39H1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB700 HL1gR4
Plasmid#124450PurposeExpresses gRNA HL1 gR4 in the pSB700 backboneDepositorInsertHL1 gR4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromotersyntheticAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N3-SLC35A2-GFP
Plasmid#186284Purposetransient expression of C-terminal GFP tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEC581
Plasmid#174369PurposeRFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.DepositorInsertRFP with PAM rich sequence upstream promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23117Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SlugBE4max
Plasmid#163799PurposeExpresses AncBE4max and nSlugCas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationSlugCas9 (D10A)PromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry_eDHFR_SIM#1
Plasmid#164646Purposetag SIM with mCherry and eDHFRDepositorInsertSIM
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgMUC4_dual-MTS-3x-Set I
Plasmid#162762PurposeSeparately expressing 3 unique sgMUC4_dual-MTS (SetI)DepositorInsertsgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#164046PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA geneDepositorInsertmTagBFG-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
IDP466
Plasmid#87008Purposegenomic Sindbis virus plasmid for expression of SYNseq componentsDepositorInsertsmyc-3xFlag-CLIP-Nrx1B-1xλN(i)
eGFP
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8792
Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AktAR
Plasmid#123356PurposeFRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.DepositorInsertLyn-AktAR
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB9337 (pgRNA_IV-1_NatMX)
Plasmid#161590PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-CLIP-FLAG-CD59
Plasmid#50375PurposeExpress CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cellsDepositorInsertCLIP
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IDP425
Plasmid#87022Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
JC101: pMVP/Ad/PK-DEST
Plasmid#121845PurposepMVP destination vector, empty pK fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8WTmneo
Plasmid#69609PurposeExpresses a neomycin tagged wildtype L1PA8DepositorInsertL1PA8
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR-SIMvada
Plasmid#164649PurposeSIM mutant tagged with mCherry and eDHFRDepositorInsertSIMvada
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLC-239
Plasmid#73193PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-yhaV (yhaV )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
jk107
Plasmid#87007Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GTH-r
Plasmid#81106PurposeGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIBR002
Plasmid#177665PurposeSIBR Intron 2 (Int2) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAID-CMV linearizing in pX330
Plasmid#140609PurposeCrispr/Cas9 plasmid for linearization of pAID-CMVDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDP427
Plasmid#87021Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-tdMS2(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hU6-mU6-Cre2GO
Plasmid#136907PurposeLentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.DepositorInsertBlasCyGO
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
IO001: pMVP (R4-R3) eGFP
Plasmid#121730PurposepMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/WT SPAST with 5'UTR
Plasmid#87731PurposeExpresses WT spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTG02-Amp-FRT
Plasmid#128271PurposePlasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)DepositorInsertTetR-LOV-RRRG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA31
Plasmid#158509PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rpp20-C1
Plasmid#134541PurposeExpresses YFP-Rpp20 fusion proteinDepositorInsertRpp20
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
