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Showing: 8751 - 8800 of 23360 results

  5. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  16. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

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  18. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDPGH023
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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  21. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  24. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  25. pDN-Cherry-sgRNA

    Plasmid
    #221387
    Purpose
    episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs
    Depositor
    Deborah Hung
    Article
    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523
    Insert
    none, cloning vector for introduction of targeting sequences
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221387-plasmid-map-sequence-id-440176

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  28. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  33. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  41. pAAV-SCP1-dSa-VPR mini.

    Plasmid
    #99685
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    99685-plasmid-map-sequence-id-194598

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  46. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74707-plasmid-map-sequence-id-152737

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  50. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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Showing: 8751 - 8800 of 23360 results