159,557 results

1. Fuw-dCas9-Dnmt3a

   Plasmid

   #84476

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Rx3(A7)

   Plasmid

   #35525

   Depositor

   Robert Cohen

   Article

   Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

   Insert

   His6-Rx3(A7)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Changed G2 to P

   Promoter

   T7

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-c4X

   Plasmid

   #75288

   Purpose

   Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 19, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLminP_Luc2P_RE29

   Plasmid

   #90371

   Purpose

   SREBF1 - Sterol gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SREBPb

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-TRE3G-PYL1-sfGFP-PML

   Plasmid

   #167011

   Purpose

   expression of PYL1-SfGFP-PML, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   PML (PML Human)
   

   Use

   Lentiviral

   Tags

   GFP-PML

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA4008

   Plasmid

   #70109

   Purpose

   mtDNA destruction/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   Herpes simplex virus UL12.5M185
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   N-terminal deletion of UL12.5

   Promoter

   CMV and RSV

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pm-iATPSnFR1.0

   Plasmid

   #102548

   Purpose

   membrane displayed ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYS16

   Plasmid

   #202082

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   CT mNG-T2A-PuroR Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-ALFANb-GB1-CAAX-WPRE

    Plasmid

    #231599

    Purpose

    Express ALFANb-CAAX

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    ALFANb-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-rNFM

    Plasmid

    #32907

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.

    Insert

    EGFP-rat NFM fusion (Nefm Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-7 asyn S129E

    Plasmid

    #36050

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. APOH-bio-His

    Plasmid

    #52177

    Purpose

    Expresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    APOH (APOH Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-ZDHHC11

    Plasmid

    #231659

    Purpose

    Expresses human ZDHHC11 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC11 (ZDHHC11 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_BiSSTe10_ChR2_mCherry

    Plasmid

    #213815

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSKDuet01

    Plasmid

    #12172

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    NpuDnaE
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFPL-Gg

    Plasmid

    #61653

    Purpose

    Gateway vector for generating green fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

    Plasmid

    #84467

    Purpose

    Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRAM and TRE

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBiFC-PBZ-VN

    Plasmid

    #110646

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus N-terminus

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Use

    Tags

    DDK and VN

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBiFC-PBZ-VC

    Plasmid

    #110648

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus C-terminus

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Use

    Tags

    HA and VC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MAC_N_KLF6

    Plasmid

    #167772

    Purpose

    Expresses KLF6 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    KLF6 (KLF6 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB4906

    Plasmid

    #106150

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL410_Ins2500

    Plasmid

    #49058

    Purpose

    Expresses luciferase under control of mouse insulin 2 gene promoter

    Depositor

    Kevin Ferreri

    Article

    Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

    Insert

    mouse Ins2 promoter (Ins2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ins2 promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/cyto-iAspSnFR

    Plasmid

    #201394

    Purpose

    CMV overexpression of cyto-iAspSnFR in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

    Insert

    cyto-iAspSnFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

    Promoter

    CMV

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK232 (CMV-OsTIR1-PURO)

    Plasmid

    #72834

    Purpose

    Expresses OsTIR1 under the control of the CMV promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-CMV-OsTIR1-PURO-AAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SNM-Luc

    Plasmid

    #35156

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 502 bp of the myc promoter

    Promoter

    c-myc

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.2-hBIN1-V5

    Plasmid

    #27303

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pFastBac-His-Tipin

    Plasmid

    #22891

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pTSAR3.5t

    Plasmid

    #210257

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    eBFP2
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTSAR3.4t

    Plasmid

    #210256

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCherry
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Ami Bhatt, Lacra Bintu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV_hSyn_WiChR_TS_mScarlet_ER

    Plasmid

    #205997

    Purpose

    High efficient, K+ selective channelrhodopsin for the inhibition of neurons

    Depositor

    Peter Hegemann, Johannes Vierock

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Wobblia Inhibitory Channelrhodopsin
    

    Use

    AAV

    Tags

    TS, mScarlet, ER

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Use

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCB1532H-RFP

    Plasmid

    #101855

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Article

    Pennington et al Figshare (2017)

    Type

    Empty backbone

    Use

    Fungal expression plasmid, tested in magnaporthe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRU43

    Plasmid

    #167665

    Purpose

    ENTRY vector for gRNA3 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge