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We narrowed to 24,790 results for: Spr

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Showing: 9051 - 9100 of 24790 results

  1. pSRF.1.0-gDNA

    Plasmid
    #112458
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SRF
    Depositor
    Kevin White
    Insert
    SRF (SRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112458-plasmid-map-sequence-id-230474

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  2. pDEAF1.2.1-gDNA

    Plasmid
    #112449
    Purpose
    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1
    Depositor
    Kevin White
    Insert
    DEAF1 (DEAF1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112449-plasmid-map-sequence-id-230462

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  3. pDLX1.1.0-gDNA

    Plasmid
    #112439
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1
    Depositor
    Kevin White
    Insert
    DLX1 (DLX1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112439-plasmid-map-sequence-id-230396

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  4. pHBP1.1.0-gDNA

    Plasmid
    #112432
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1
    Depositor
    Kevin White
    Insert
    HBP1 (HBP1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112432-plasmid-map-sequence-id-230372

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  5. pATF1.1.0-gDNA

    Plasmid
    #112424
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1
    Depositor
    Kevin White
    Insert
    ATF1 (ATF1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112424-plasmid-map-sequence-id-230304

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  6. dSp-Rta 100-175

    Plasmid
    #99667
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (101-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    99667-plasmid-map-sequence-id-192044

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  7. dSp-Rta 50-175

    Plasmid
    #99665
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (51-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    99665-plasmid-map-sequence-id-192035

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  8. pAW218

    Plasmid
    #113240
    Purpose
    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip
    Depositor
    Jennifer Doudna
    Article
    Wright et al Science. 2017 Sep 15;357(6356):1113-1118
    Inserts
    Cas1
    Cas2
    Leader-repeat
    Expression
    Bacterial
    Mutation
    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113240-plasmid-map-sequence-id-217755

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  19. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1TDPGH023
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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  34. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  36. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  40. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  45. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

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  48. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

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Showing: 9051 - 9100 of 24790 results