We narrowed to 9,415 results for: Coli

1. pVRa15_436

   Plasmid

   #49667

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF15_436
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVRa28_1088

   Plasmid

   #49683

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF28_1088
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DT050A

   Plasmid

   #159411

   Purpose

   Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   J23101 promoter

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVRa11_987

   Plasmid

   #49661

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF11_987
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

   Plasmid

   #61938

   Purpose

   Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF2) (UCHL5 Human)
   hNFRKB (39-156) (NFRKB Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156, residues 2-38 dele…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET151-GFP-PP-RPN2 (925-953)

   Plasmid

   #73744

   Purpose

   Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pVRa15_524

   Plasmid

   #49668

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF15_524
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET151-GFP-PP-RPN2 (918-932)

   Plasmid

   #73748

   Purpose

   Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-932 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pVRa17_1691

   Plasmid

   #49672

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF17_1691
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28b-hTIG3-1-164

    Plasmid

    #100724

    Purpose

    Full-length human TIG3 in E.coli expression vector

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    RARRES3 (PLAAT4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVRa37_2513

    Plasmid

    #49696

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF37_2513
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28b-hTIG3-1-132

    Plasmid

    #100720

    Purpose

    Expression of N-terminal region of human TIG3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    RARRES3 (PLAAT4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRa07_980

    Plasmid

    #49659

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF07_980
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRa30_83

    Plasmid

    #49686

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF30_83
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

    Plasmid

    #61943

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Residues 2-39 deleted resulting in construct star…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pVRa16_973

    Plasmid

    #49669

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF16_973
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRa11_3726

    Plasmid

    #49662

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF11_3726
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEvol-chKlacRS-IPYE

    Plasmid

    #232160

    Purpose

    A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    chKlacRS-IPYE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. arginine biosensor cpFLIPR

    Plasmid

    #205746

    Purpose

    Robust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp

    Depositor

    Colin Jackson

    Article

    Whitfield et al Protein Sci. 2015 Jun 9. doi: 10.1002/pr

    Insert

    cpFLIPR
    

    Use

    Unspecified

    Tags

    6 x Histidine purification tag (N-terminal), Biot…

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-parkin W403A

    Plasmid

    #227357

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin W403A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-parkin L107K

    Plasmid

    #227363

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin L107K (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET30-10His-FKBP(f36v)-SNAP

    Plasmid

    #200282

    Purpose

    For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    FKBP-SNAPtag
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-parkin R104A

    Plasmid

    #227358

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin R104A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-parkin Ubl P37L

    Plasmid

    #227366

    Purpose

    For bacterial expression of mutated parkin Ubl.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin 1-76 P37L (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-parkin D106A

    Plasmid

    #227362

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin D106A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-parkin V105K

    Plasmid

    #227361

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin V105K (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-parkin T103A

    Plasmid

    #227360

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin T103A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-parkin L102K

    Plasmid

    #227359

    Purpose

    For bacterial expression of parkin with a mutated ACT.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin L102K (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-parkin M458L

    Plasmid

    #227356

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin M458L (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-parkin R256C

    Plasmid

    #227355

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin R256C (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-parkin R234Q

    Plasmid

    #227354

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin R234Q (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-parkin V224A

    Plasmid

    #227353

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin V224A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-parkin P37L

    Plasmid

    #227352

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin P37L (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSF_ApyADO

    Plasmid

    #226248

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSF_ApyAO

    Plasmid

    #226242

    Purpose

    Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSF_ApyAD

    Plasmid

    #226241

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pECNUS2

    Plasmid

    #184885

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHis-WzzE

    Plasmid

    #196661

    Purpose

    Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGST-WzzE

    Plasmid

    #196662

    Purpose

    Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable GST

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2.

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHis-mCherry-WzzE

    Plasmid

    #196663

    Purpose

    Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2 of WzzE.

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPglL-strep-mCherry

    Plasmid

    #196664

    Purpose

    Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable distrep tag II and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPglL-strep-mClover3

    Plasmid

    #196665

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pWzyE-GST-distrepII

    Plasmid

    #196666

    Purpose

    Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    GST and TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pWzyE-strep-mClover3

    Plasmid

    #196668

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pECNUS3

    Plasmid

    #184886

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pECNUS1

    Plasmid

    #184884

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. DT033A

    Plasmid

    #159394

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET151-GST-PP-RPN13 (19-132) K97E

    Plasmid

    #125825

    Purpose

    Expresses GST-RPN13 (19-132) K97E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 97 to glutamate

    Promoter

    T7

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET151-GST-PP-RPN13 (19-132) K83A

    Plasmid

    #125829

    Purpose

    Expresses GST-RPN13 (19-132) K83A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 83 to alanine

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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