We narrowed to 31,552 results for: Eng;

1. pSDM177

   Plasmid

   #194476

   Purpose

   pHERD30T-crRNA(Lse)_V3

   Depositor

   Joseph Bondy-Denomy

   Article

   Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

   Insert

   crRNA(Lse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-jAspSnFR3

   Plasmid

   #203488

   Purpose

   jAspSnFR3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTAT-HA

   Plasmid

   #35612

   Depositor

   Steven Dowdy

   Article

   Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

   Type

   Empty backbone

   Use

   Tags

   6xHIS, HA, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ144

   Plasmid

   #69296

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBLO 9.3_bCas9-CP230

   Plasmid

   #121497

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3.3TOPO-T7-hCas9

   Plasmid

   #161876

   Purpose

   In vitro transcription of SpCas9

   Depositor

   Stephane Pelletier

   Article

   Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

   Insert

   human codon optimized SpCas9
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR_AXP_hrGFP

   Plasmid

   #84613

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYD1-mSA

   Plasmid

   #39865

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin
   

   Use

   Tags

   Aga2, FLAG, and HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTFUbiCre

   Plasmid

   #139670

   Purpose

   Expression cassette for Cre recombinase in T-DNA vector

   Depositor

   James Birchler

   Article

   Gaeta et al Chromosoma. 2013 Jun;122(3):221-32. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEV-G3-Km

    Plasmid

    #46817

    Purpose

    TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and GAL10

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NGFR

    Plasmid

    #27489

    Depositor

    Warren Pear

    Article

    Izon et al Immunity. 2001 Mar . 14(3):253-64.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    ECMV, IRES, and NGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ141A

    Plasmid

    #69290

    Purpose

    Gateway entry vector; single gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW019-2

    Plasmid

    #85614

    Purpose

    Inducible dCas9 integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PxylA (B. megaterium)

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTHSSe_50

    Plasmid

    #109248

    Purpose

    incW BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMZ0002

    Plasmid

    #102239

    Purpose

    Expresses Chinese hamster NSF in bacteria

    Depositor

    Axel Brunger

    Article

    Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

    Insert

    N-ethylmaleimide sensitive factor
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSN6

    Plasmid

    #131318

    Purpose

    Integrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassa

    Depositor

    Eugene Gladyshev

    Article

    Nguyen et al Fungal Genet Biol. 2020 Mar;136:103316.

    Insert

    Full-length tetO array (XbaI-NheI fragment of pLAU44)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKL2315

    Plasmid

    #187876

    Purpose

    Expresses Cre recombinase.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. haA1.pBABEpu

    Plasmid

    #21813

    Depositor

    David Ron

    Article

    Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

    Insert

    GADD34 C-term
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLPC myc hPOT1

    Plasmid

    #12387

    Depositor

    Titia de Lange

    Article

    Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

    Insert

    Protection of Telomeres 1 (POT1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR_MFE1_hrGFP

    Plasmid

    #84617

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAW014-2

    Plasmid

    #85612

    Purpose

    Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    ugtP-gRNA.P395T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSET his-mCherry

    Plasmid

    #113552

    Purpose

    For bacterial expression and purification of mCherry

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    his-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR_A08_hrGFP

    Plasmid

    #84615

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHK352.3

    Plasmid

    #235473

    Purpose

    Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    sgRNA3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p361_DG

    Plasmid

    #234683

    Purpose

    Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR_CMV_Melt-30-mCherry

    Plasmid

    #234061

    Purpose

    Encodes a Melt variant with a switching temperature of 30°C

    Depositor

    Lukasz Bugaj

    Article

    Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

    Insert

    Melt-30-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid

    #229617

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R337Q

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid

    #229618

    Purpose

    Mammalian expression of full length mouse FoxP3 (A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A372S

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FBE/SBE-Luc

    Plasmid

    #16525

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FBE/SBE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

32. pXP220

    Plasmid

    #26832

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNJB80

    Plasmid

    #51518

    Purpose

    Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GLI2 his

    Plasmid

    #87097

    Purpose

    expresses GLI2 in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x His, T7 tag, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMEV4

    Plasmid

    #110539

    Purpose

    The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

    Depositor

    William B. Whitman

    Article

    Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCS2-XTbx5-EnR

    Plasmid

    #117249

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5-EnR (tbx5-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    chimeric protein with engrailed repressor domain…

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHR_EF1a-->GFPlig

    Plasmid

    #162223

    Purpose

    Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJH124

    Plasmid

    #102951

    Purpose

    Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

    Depositor

    Peter Espenshade

    Article

    Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

    Insert

    APEX2-NES
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ141C

    Plasmid

    #69292

    Purpose

    Gateway entry vector; single gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. lacI-PT7-gusA-pBAV1k

    Plasmid

    #30504

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucoronidase (gusA Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge