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pWPT-PDX1

Plasmid

#12256

Depositor

Didier Trono

Article

Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

Insert

Pdx1 (PDX1 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#190163

Purpose

Microglia-targeted transgene expression.

Depositor

Hirokazu Hirai

Article

Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p

Use

AAV

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSin Capsid

Plasmid

#127693

Purpose

Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Capsid

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSpCas9(BB)-2A-Hygro

Plasmid

#127763

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available Since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57.1-FOXM1b

Plasmid

#68811

Purpose

Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1b (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

HA

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE3

Plasmid

#61622

Purpose

FRET-based biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

ICUE3 (RAPGEF3 Human)

Tags

ECFP and cpVenus[L194]

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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