171,965 results
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Plasmid#61109PurposeExpression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fieldsDepositorInsertspectrin-cpstFRET
ExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha
Plasmid#62520Purposewild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sitesDepositorAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
cpLOV2-V7
Plasmid#168993Purposecircularly permuted LOV2 variant-V7DepositorInsertcpLOV2-V7
ExpressionMammalianPromoterCMVAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3
Plasmid#172922PurposeFor enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV1 and AAV9Insertaxon-jGCaMP8m-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain (N terminal on insert…ExpressionMammalianPromoterhSynapsin1Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro
Plasmid#176851Purposelentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expressionDepositorInserthYAP1-5SA (YAP1 Human)
UseLentiviralMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…Available SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Hygro
Plasmid#25737Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHIV-SapI-LacZ
Plasmid#237996PurposeExpression plasmid for transfer of complete TCR library from pGGA backboneDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
smHA multi-frame tag
Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
ExpressionMammalianAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM427
Plasmid#210788PurposeL5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transferDepositorInsertDefective hygromycin resistant gene
ExpressionBacterialMutationChanged hygromycin resistant gene W190 codon (TGG…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHaMDRwt
Plasmid#10957DepositorAvailable SinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE
Plasmid#188946PurposeLevel 1 destination vector containing pBBR1 origin of replication and CIDAR AE scarsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-STAT1-mGFP-P2A-Puro
Plasmid#199345Purposeexpress murine STAT1 protein fused with mGFPDepositorInsertsignal transducer and activator of transcription 1 (Stat1 Mouse)
UseLentiviralTagsmGFPExpressionMammalianPromoterCMVAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAktKAR2.2-T2A-EGFP
Plasmid#216494PurposeExpression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAktKAR2.2
TagsT2A-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OSKM
Plasmid#80481PurposepiggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianPromotertetOAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-NRASG12D-BFP
Plasmid#236202PurposeExpresses NRASG12D in mammalian cellsDepositorAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Lifeact-7
Plasmid#54586PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertLifeact-mTagRFP-T
ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorAvailable SinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#201157PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a-VP64
UseCRISPR and LentiviralMutationD908APromoterEFSAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)
Plasmid#85583PurposecGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)DepositorInsertPrab-3-GAL4-SK(DBD)-VP64
ExpressionWormPromoterPrab-3-GAL4-SK(DBD)-VP64Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA1_D262V-FLAG-IRES-eGFP
Plasmid#234620PurposeMammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequenceDepositorInserthnRNPA1 D262V (HNRNPA1 Human)
TagsFLAGExpressionMammalianMutationFamillial ALS mutation D262V, C-terminal FLAG tag…PromoterCMVAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_HNF1A-RE_tBFP_PGK_mCitrine
Plasmid#188387PurposeLentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;DepositorInsertHNF1A-RE_tBFP_PGK_mCitrine
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABE8e
Plasmid#138493PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (CP1041)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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hKCNQ5(WT).ires.GFP-pcDNA3
Plasmid#204360PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pY010 (pcDNA3.1-hAsCpf1)
Plasmid#69982PurposeExpresses humanized AsCpf1DepositorInserthAsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128589PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterSynAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM47523
Plasmid#153221PurposeLevel 0 zCas9i vectorDepositorInsertSpCas9 with introns and 2 NLS
UseSynthetic BiologyAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
5KKP (PUS7)
Plasmid#101878PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-NLS-mCherry
Plasmid#231890PurposemCherry-tagged nuclear rupture markerDepositorInsertmCherry-SV40 NLS-mCherry
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TYRO3-V5/HIS
Plasmid#200118PurposeExpression of human TYRO3 receptor tyrosine kinase in mammalian cellsDepositorInserthuman TYRO3 receptor tyrosine kinase (TYRO3 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCnCas12f1HS_sgRNA_MS13_empty
Plasmid#204999PurposeCnCas12f1 with MS13 sgRNA site for Human cell genome editingDepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Left
Plasmid#52341PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Left
ExpressionMammalianPromoterCMVAvailable SinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
B108-GFP-IRES-AP
Plasmid#73988PurposeB108 enhancer of Blimp1 gene drives EGFP expression.DepositorInsertB108 enhancer
ExpressionMammalianAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα _WPRE
Plasmid#196675PurposeAAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).DepositorInserttAKARα
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#226514PurposeMamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2DepositorInsertHaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-PIMT
Plasmid#34852DepositorAvailable SinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5_mCherry-P300
Plasmid#238258PurposeFor overexpression of mCherry-P300DepositorInsertmCherry-P300
ExpressionMammalianAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPH2
Plasmid#19535DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
TagsHisExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…Available SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertVillin-mTQ2-P2A-FlpO-ERT2
TagsmTQ2, linked to FlpO through an P2A ribosomal ski…ExpressionMammalianPromoterVillinAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP-Casp3
Plasmid#81241PurposeA ZipGFP-based Caspase-3 protease reporter T2A mCherryDepositorInsertZipGFP with caspase cleavage sequence T2A mCherry
ExpressionMammalianAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-EGFP-WPRE-pA
Plasmid#37084PurposeCre-dependent expression of EGFPDepositorInsertEGFP
UseAAV and Cre/Lox; Cre-onPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only