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61,387 results

Showing: 9401 - 9450 of 61387 results

  7. pLV-SFFV-CALU-WPRE-UbC-mCherry

    Plasmid
    #225952
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CALU (CALU Human)
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225952-plasmid-map-sequence-id-446855

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  9. pInducer 21-3xFlag-hMEFV-S580X

    Plasmid
    #195474
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed S580 to a stop codon
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195474-plasmid-map-sequence-id-386691

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  17. TFORF0499

    Plasmid
    #143881
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARGC1A (PPARGC1A Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143881-plasmid-map-sequence-id-404556

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  25. MELOPS

    Plasmid
    #80151
    Purpose
    Genetically encoded melanosome-localized pH sensor.
    Depositor
    Santiago Di Pietro
    Article
    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11
    Insert
    OCA2 (OCA2 Human)
    Tags
    mNectarine in first luminal loop
    Expression
    Mammalian
    Mutation
    nonpathologic polymorphism D257A, pore mutation V…
    Promoter
    CMV
    Available Since
    Sept. 20, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid
    #135267
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135267-plasmid-map-sequence-id-269144

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  30. HA-Clover-HIF-2alpha Wildtype

    Plasmid
    #163371
    Purpose
    Express HA- and Clover-tagged HIF-2alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163371-plasmid-map-sequence-id-322295

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  31. TFORF0122

    Plasmid
    #141527
    Purpose
    Lentiviral vector for overexpressing the BARX1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BARX1 (BARX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141527-plasmid-map-sequence-id-276717

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  32. MC4R-Tango

    Plasmid
    #66430
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC4R (MC4R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66430-plasmid-map-sequence-id-314166

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  33. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  38. pRPA70-IRES-Ven-RPA32-IRES-RPA14

    Plasmid
    #211430
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Tags
    mVenus (on RPA32)
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211430-plasmid-map-sequence-id-420414

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  39. TFORF0253

    Plasmid
    #143208
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CAMTA1 (CAMTA1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143208-plasmid-map-sequence-id-415861

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  40. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  41. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164786-plasmid-map-sequence-id-326679

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  45. PTH2R-Tango

    Plasmid
    #66490
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    PTH2R (PTH2R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156643
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIR2DL5A (KIR2DL5A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156643-plasmid-map-sequence-id-430447

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Showing: 9401 - 9450 of 61387 results