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Showing: 901 - 950 of 9506 results

  3. Ydf NanoLuc

    Plasmid
    #225656
    Purpose
    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NanoLuciferase
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Promoter
    PydfJ115
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225656-plasmid-map-sequence-id-455061

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  6. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid
    #216379
    Purpose
    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Frizzled-4 (Fzd4 Mouse)
    Use
    Baculovirus
    Tags
    6xHis and GP64 signal sequence
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216379-plasmid-map-sequence-id-433261

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  9. GST-prRDH-CTS

    Plasmid
    #86069
    Purpose
    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Retinol dehydrogenase (RDH8 Bovine)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    the insert is 297-312 of RDH8 (Bos taurus)
    Promoter
    tac
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86069-plasmid-map-sequence-id-177979

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  29. pAL149

    Plasmid
    #22275
    Purpose
    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB(1-908) (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    mCherry-Kras4BCT (prenylation sequence)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    22275-plasmid-map-sequence-id-207638

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  33. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Arthur Weiss, Lawrence Kane
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Promoter
    Available since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    CD58 c-term (CD58 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

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  36. pAL204

    Plasmid
    #22282
    Purpose
    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2010
    Availability
    Academic Institutions and Nonprofits only
    22282-plasmid-map-sequence-id-80604

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  41. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid
    #207148
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI1 (GNAI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207148-plasmid-map-sequence-id-410277

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  43. pGEM-PH-citrine

    Plasmid
    #131406
    Purpose
    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
    Depositor
    Lars Hufnagel
    Article
    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369
    Insert
    PH
    Use
    In vitro transcription
    Tags
    citrine yellow fluorescent protein
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131406-plasmid-map-sequence-id-261478

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  45. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  50. GST-PC-2-CTS

    Plasmid
    #86068
    Purpose
    A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Polycystin-2 (Pkd2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains the region from 1-15
    Promoter
    tac
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86068-plasmid-map-sequence-id-177978

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Showing: 901 - 950 of 9506 results