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Addgene

We narrowed to 2,414 results for: NIG

Showing: 901 - 950 of 2414 results
  1. pFlp-Km

    Plasmid
    #222334
    Purpose
    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3
    Depositor
    Inserts
    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    Use
    Tags
    Expression
    Mutation
    Promoter
    cl, flp, and sacB
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pMP71-super-IL-2

    Plasmid
    #174599
    Purpose
    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab
    Depositor
    Insert
    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pLyGo-Ec-7

    Plasmid
    #163135
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Nanobody, C-IgAP autotransporter
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. SnFR-gamma2

    Plasmid
    #165495
    Purpose
    Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.
    Depositor
    Insert
    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pMflET-o4

    Plasmid
    #101314
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pMflT-o4

    Plasmid
    #101312
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Insert
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pMflPT-o4

    Plasmid
    #101315
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 901 - 950 of 2414 results