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173,773 results

Showing: 9751 - 9800 of 173773 results

  2. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  7. pR_LG lenti-(empty)-del-CTS

    Plasmid
    #112895
    Purpose
    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries
    Depositor
    Paul Blainey
    Article
    Feldman et al bioRxiv 262121
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    none
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112895-plasmid-map-sequence-id-217829

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  10. pcDNA_mIGg2a_Anti-HA [12CA5] v2

    Plasmid
    #236295
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK_Anti-HA [12CA5] v2 light chain (Plasmid 236296) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a Fc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236295-plasmid-map-sequence-id-467832

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  11. pcDNA_mK_Anti-HA [12CA5] v2

    Plasmid
    #236296
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-HA [12CA5] v2 heavy chain (Plasmid 236295) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] light chain variable region fused to mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236296-plasmid-map-sequence-id-467834

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  13. pCAG-N2cG

    Plasmid
    #100811
    Purpose
    express rabies virus CVS N2c strain glycoprotein gene (N2cG)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain glycoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    100811-plasmid-map-sequence-id-189508

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  17. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  18. HA-HA-AR-NTD

    Plasmid
    #171235
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171235-plasmid-map-sequence-id-336028

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  20. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  23. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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  32. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

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  34. pQCXIP-VEGFC

    Plasmid
    #73012
    Purpose
    Retroviral expression of human VEGFC
    Depositor
    Michael Grusch
    Insert
    VEGFC (VEGFC Human)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73012-plasmid-map-sequence-id-133866

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  37. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

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  40. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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Showing: 9751 - 9800 of 173773 results