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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSL2680

Plasmid

#85581

Purpose

Replicating base vector for constructing cpf1/CRISPR editing plasmids

Depositor

Himadri Pakrasi

Article

Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E542K

Plasmid

#116479

Purpose

Lentiviral expression of PIK3CA E542K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E542K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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U6-5' MS2-CircRNA

Plasmid

#213750

Purpose

For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pUltra

Plasmid

#24129

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

Depositor

Malcolm Moore

Article

Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-hERalpha

Plasmid

#101141

Purpose

Mammalian expression of full-length human estrogen receptor-alpha

Depositor

Elizabeth Wilson

Article

Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

Insert

estrogen receptor-alpha (ESR1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2s

Plasmid

#231763

Purpose

Expression of mGold2s in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Mammalian

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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DsRed2-Mito-7

Plasmid

#55838

Purpose

Localization: Mitochondria, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

DsRed2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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PB533_15F11-sfGFP

Plasmid

#167526

Purpose

H4K20me1-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

Insert

15F11-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-cfGFP

Plasmid

#200773

Purpose

Expresses cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

cysteine-free GFP

Use

AAV

Promoter

Synapsin

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Promoter

lac

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UnReg-H2B-YFP-DD

Plasmid

#96892

Purpose

Reporter to monitor translation of unstructured mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

UnReg-H2B-Venus-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

Plasmid

#50468

Purpose

Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

Depositor

Bryan Roth

Insert

rM3D-IRES-mCitrine

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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NanoLuc-CCNE1 Vector

Plasmid

#238665

Purpose

Express NanoLuc-CCNE1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CCNE1 (CCNE1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N EBNA3C

Plasmid

#37958

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA3C (EBNA-3B/EBNA-3C H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5 (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

Plasmid

#223747

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Lambda-PPase

Tags

6xHis-TEV

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-M2

Plasmid

#44170

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Influenza A virus M2 matrix gene

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pBPLexA::p65Uw

Plasmid

#26231

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

LexA::p65 (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9_GFP

Plasmid

#44719

Purpose

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Mem

Plasmid

#55779

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCherry-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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