162,536 results
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pBFC0993
Plasmid#231993PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacerDepositorInsertsdRfxCas13d
crRNA with negative control (RFP-targeting) spacer
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and pTetAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.MTF2
Plasmid#125169PurposeExpresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertMTF2 (MTF2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-SHMT2-3xFLAG
Plasmid#233891Purposelentiviral overexpression vector for SHMT2DepositorInsertSHMT2 (SHMT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-mStayGold-GB1-dWPRE
Plasmid#231598PurposeExpress scFv_GCN4-mStayGold for SunTag translation imagingDepositorInsertscFv-GCN4-mStayGold
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209665PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mNaChBac-T2A-tdTomato
Plasmid#60650PurposeExpresses mNaChBac-T2A-tdTomatoDepositorInsertmNaChBac-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_EV
Plasmid#183116PurposeEmpty vector control for pLenti_EF1a constructsDepositorInsertSmall multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPar2
Plasmid#53228Purposemammalian expression of human Par2DepositorInsertPar2 (F2RL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1alpha
Plasmid#44224DepositorInsertPP1A (PPP1CA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pPB-US-ECasE
Plasmid#83961PurposePiggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.DepositorInsertERT2-spCas9-ERT2
UseTagsExpressionMammalianMutationplease see depositor comments belowPromoterU6 (gRNA), EF1a (ERT2-Cas9-ERT2)Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMTMVi-M
Plasmid#118757PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NUP50-N-10
Plasmid#55111PurposeLocalization: Nuclear Pore, Excitation: 587, Emission: 610DepositorInsertNUP50 (NUP50 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA
Plasmid#136537PurposeLentiviral expression vector for an inducible BirADepositorInsertBirA
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Cdc42-2G
Plasmid#68812PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)DepositorInsertCdc42 activation reporter
UseAdenoviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC2-pA
Plasmid#220754PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC1-pA
Plasmid#220753PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC1
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHuji (CD63 Human, synthetic)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-Sec61 beta
Plasmid#49155Purposeexpression of Sec61 beta fused to mCherry, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsmCherry*ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)
Plasmid#169188PurposeTo express His-SUMO-nsp12 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDH2-WT
Plasmid#87926PurposeExpresses IDH2 (wild-type) in mammalian cellsDepositorInsertisocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61β
Plasmid#172445PurposeExpression of ER protein Sec61beta with an N-terminal mCherry tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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hSUMO1 GFP
Plasmid#118360PurposeThis plasmid expresses the full length human SUMO1.DepositorInserthSUMO1 (SUMO1 Human)
UseTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.1
Plasmid#208100PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOs
Plasmid#189931PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-U
Plasmid#51230Purposebi-directional expression vector for yeast, constitutively active, URA3 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBI-p53 R175H/EGFP
Plasmid#16544DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationR175HPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR2 (CXCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-D
Plasmid#38133DepositorInsertMITF-D (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Fascin-C-10
Plasmid#54094PurposeLocalization: Actin Bundling Protein, Excitation: 487, Emission: 509DepositorInsertFascin (FSCN1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC-LaminA
Plasmid#69059Purposeencodes the lamin A proteinDepositorInsertLaminA (LMNA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT85_PS-mEos3.2_GentaR
Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
UseTagsExpressionBacterialMutationPromoterSpiralinAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P29
Plasmid#120040PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK2
Plasmid#122673PurposeExpresses mouse PCSK2 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0335
Plasmid#144358PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF91 (ZNF91 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAT3(Y705F)-TAL-Luc
Plasmid#46933PurposeLuciferase Reporter for mutant STAT3DepositorInsertSTAT3(Y705F) (STAT3 Human)
UseLuciferaseTagsFLAGExpressionMutationTyrosine 705 to PhenylalaninePromoterTA LuciferaseAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLIB
Plasmid#80610PurposepLIB - Library vector (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Eomes
Plasmid#70271PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine EomesDepositorInsertEomes (Eomes Mouse)
UseTagsExpressionMutationPromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag
Plasmid#209666PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-ERT2CreERT2
Plasmid#149436PurposeROSA26 targetting vector with tamoxifen-inducible Cre recombinaseDepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
