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Showing: 951 - 1000 of 4068 results

  11. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid
    #238344
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96E; R103E; R105E
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238344-plasmid-map-sequence-id-472712

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  12. Ec FkP-dnaN(3A's)-pRSFDuet

    Plasmid
    #238343
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96A; R103A; R105A
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238343-plasmid-map-sequence-id-472707

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  13. Ec FkP-dnaN(3S's)-pRSFDuet

    Plasmid
    #238345
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    R96S; R103S; R105S
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238345-plasmid-map-sequence-id-472709

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  19. pRS414-Syf1 817 Truncation

    Plasmid
    #234126
    Purpose
    Syf1 PCR truncation starting at 817 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234126-plasmid-map-sequence-id-466534

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  20. pRS414-Syf1 634 Truncation

    Plasmid
    #234116
    Purpose
    Syf1 PCR truncation starting at 634 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234116-plasmid-map-sequence-id-466457

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  21. pRS414-Syf1 778 Truncation

    Plasmid
    #234115
    Purpose
    Syf1 PCR truncation starting at 778 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234115-plasmid-map-sequence-id-465993

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  29. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  30. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  31. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid
    #221160
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221160-plasmid-map-sequence-id-450956

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  32. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

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  33. pRSF-DUET-C17orf53_1-87-Strep

    Plasmid
    #211433
    Purpose
    bacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    Strep-Tag
    Expression
    Bacterial
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211433-plasmid-map-sequence-id-420530

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  34. pRSF-Duet-C17orf53-6His

    Plasmid
    #211417
    Purpose
    bacterial expression of human C17orf53 C-terminally fused to 6xHis-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211417-plasmid-map-sequence-id-420520

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  35. pRSF-Duet-6His-C17orf53

    Plasmid
    #211416
    Purpose
    bacterial expression of human C17orf53 N-terminally fused to a 6xHis-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211416-plasmid-map-sequence-id-420518

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Showing: 951 - 1000 of 4068 results