We narrowed to 17,391 results for: puro

1. p6659 MSCV-IP N FlagHA HPV16E6

   Plasmid

   #44152

   Depositor

   Peter Howley

   Article

   White et al J Virol. 2012 Dec;86(24):13174-86. doi:

   Insert

   HPV16E6
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

2. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ATM-Halo HRD

   Plasmid

   #207082

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCW-SPIC

   Plasmid

   #162825

   Purpose

   Doxycycline-inducible overexpression of human SPIC

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   SPIC (SPIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiGuide_GFP_NT135

   Plasmid

   #183118

   Purpose

   pLentiGuide modified to express GFP and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMRXIP SECFP-Stx17TM

   Plasmid

   #86777

   Purpose

   Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   transmembrane domains of human Syntaxin 17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   SECFP

   Expression

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-opto-hMLKL

   Plasmid

   #208789

   Purpose

   Inducibly expresses human MLKL, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MLKL (MLKL Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti TurboRFP BlastR

   Plasmid

   #102343

   Purpose

   Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   TurboRFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX.TRE3G.AdProt

    Plasmid

    #131687

    Purpose

    A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPC-Flag-PML-IV

    Plasmid

    #62804

    Purpose

    expresses the isoform IV of PML (promyelocytic leukemia protein)

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Express a lysosomal Tag

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Expression

    Mutation

    Promoter

    ubc

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-cT3G-cERP2-HDAC2

    Plasmid

    #222553

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HDAC2 (HDAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228362

    Purpose

    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TTVTFLPTPSLILQT_epitope

    Plasmid

    #165021

    Purpose

    Expression of CMV UL44 (38-52) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL44 (38-52)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-U6-sgRNA-BFP

    Plasmid

    #107722

    Purpose

    For expression of sgRNA from the U6 promoter with BFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDA007

    Plasmid

    #131380

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Use

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLENTI_RPS10_K138/139R

    Plasmid

    #127138

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K138R/K139R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-EF1a-hMLH1dn-eGFP

    Plasmid

    #191104

    Purpose

    Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXPR_501

    Plasmid

    #96922

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pM378

    Plasmid

    #173174

    Purpose

    Expresses C9-1 gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    C9-1 targeting gRNA-12xMBS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EcSTH

    Plasmid

    #211923

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMXs-IP GFP-WIPI-1

    Plasmid

    #38272

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXPR_003 sgMSLN

    Plasmid

    #193588

    Purpose

    MSLN knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgMSLN (MSLN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEFIRES-P-mCherry-HPos

    Plasmid

    #87159

    Purpose

    Fluorescent marker of lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    HPos
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDH-SIRT6-H133Y

    Plasmid

    #102327

    Purpose

    Human SIRT6 H133Y for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid

    #186309

    Purpose

    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg30-PDGFR
    mCherry
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLIX403-hNOS2

    Plasmid

    #110800

    Purpose

    tet-inducible NOS2 expression

    Depositor

    Edward Morgan

    Article

    Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

    Insert

    NOS2 (NOS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EcSTH-LbNOX

    Plasmid

    #211924

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH-LbNOX
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB-cT3G-cERP2-HMGB2

    Plasmid

    #222532

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HMGB2 (HMGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB-cT3G-cERP2-EZH2

    Plasmid

    #222528

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCIP-AMPKa1_WT

    Plasmid

    #79010

    Purpose

    For mammalian expression of WT AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p6618 MSCV-IP N-HAonly WHSC2

    Plasmid

    #35014

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    WHSC2 (NELFA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid

    #135281

    Purpose

    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-CDK12 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

44. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMRXIP-3xMyc-STX17FL

    Plasmid

    #89942

    Purpose

    Expresses syntaxin17 tagged with Myc in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

    Insert

    human syntaxin17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLJM1 Flag Raptor Rheb15

    Plasmid

    #26634

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor-Rheb15 (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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47. pEBTet_NADP-Snifit-mito

    Plasmid

    #113919

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. Flag pLJM1 RagB 99L

    Plasmid

    #19315

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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49. GFP-HuR

    Plasmid

    #121162

    Purpose

    Mammalian expression of GFP tagged HuR

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    HuR (ELAVL1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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