We narrowed to 22,948 results for: Tes

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pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

L107F

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pLX302-MKP7-V5 puro

Plasmid

#87771

Purpose

Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP7 (DUSP16 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV iGluf

Plasmid

#105833

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_ATRX

Plasmid

#221377

Purpose

Expresses ATRX in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Bacterial

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pVenus-Sec16A

Plasmid

#36157

Purpose

Expresses Sec16A as a Venus FP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173863

Purpose

Mammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Expression

Bacterial

Promoter

lac promoter

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-Tmx4-V5

Plasmid

#120236

Purpose

Expresses V5-tagged Tmx4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V-IRES-ASK1

Plasmid

#221074

Purpose

Expresses NRAS G12V and ASK1 in mammalian cells

Depositor

Hidenori Ichijo

Article

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Inserts

NRAS (NRAS Human)
ASK1

Expression

Mammalian

Mutation

G12V

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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DNM2 delta PRD

Plasmid

#168275

Purpose

Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

DNM2 (DNM2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa 1-742

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pU6 SgRNA GAPDH

Plasmid

#162736

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

Spacer sequence for GAPDH

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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UBQLN2_450-624W

Plasmid

#176852

Purpose

Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

Depositor

Carlos Castañeda

Article

Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

Insert

UBQLN2_450C_W (UBQLN2 Human)

Expression

Bacterial

Mutation

deletion of residues 1-449 and addition of C-term…

Promoter

T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Dynamin I-K44A-non-tagged

Plasmid

#32695

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pETDuet1-WT-LanCL2-C-Ter-His

Plasmid

#154186

Purpose

To express LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28A-mCCHC

Plasmid

#51372

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Tags

FLAG

Expression

Mammalian

Mutation

CCHC domain mutant (H147A, H169A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pHS1516

Plasmid

#205979

Purpose

FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas7 from MWEE01000013, Cas5-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
MBC1-PuroIII

Plasmid

#153419

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Rac1-2G

Plasmid

#66112

Purpose

Second generation Rac1 FRET biosensor for adenovirus production

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Adenoviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAF4

Plasmid

#58318

Purpose

EGFP-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pLV V5-Vkorc1l1

Plasmid

#175148

Purpose

Lentiviral expression of V5-tagged mouse Vkorc1l1

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Vkorc1l1 (Vkorc1l1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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AA007

Plasmid

#216002

Purpose

Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA032

Plasmid

#216012

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dCas9_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-Cas9-VPR

Plasmid

#115147

Purpose

Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-Cas9-VPR

Use

CRISPR

Tags

SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RNF110

Plasmid

#126547

Purpose

Expresses GFP-tagged RNF110 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF110 (PCGF2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS ColIII alpha1

Plasmid

#19187

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Procollagen type III alpha 1 (COL3A1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173875

Purpose

Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173876

Purpose

Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Tmem161a-V5

Plasmid

#175149

Purpose

Lentiviral expression of mouse Tmem161a-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem161a (Tmem161a Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Bnip3L RNAi pSuper-retro

Plasmid

#17468

Depositor

Wafik El-Deiry

Article

Fei et al Cancer Cell. 2004 Dec . 6(6):597-609.

Insert

Bnip3L RNAi (BNIP3L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pETM33_KEAP1_KELCH

Plasmid

#178496

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAVS1-Neo-TNNT2-Puro

Plasmid

#214016

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Neo-TNNT2-Zeo

Plasmid

#214017

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDARMO_CMVT_3xFLAG-HUWE1_R4187C

Plasmid

#187142

Purpose

mammalian cell expression of 3xFLAG-HUWE1 R4187C

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

R4187C

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TRBP-L1+D1+L2-myc/His

Plasmid

#180599

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG W96A-GLTP

Plasmid

#170741

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

W96A

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AA015

Plasmid

#216058

Purpose

Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA406

Plasmid

#216070

Purpose

Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 22,948 results for: Tes